Identification of operators and promoters that control SXT conjugative transfer
Identification of operators and promoters that control SXT conjugative transfer
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DOI：
10.1128/jb.186.17.5945-5949.2004
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发表时间：
2004-09-01
影响因子：
3.2
通讯作者：
Waldor, MK
中科院分区：
文献类型：
--
作者：
Beaber, JW;Waldor, MK
SXT是一种霍乱弧菌衍生的整合接合元件，编码多种抗生素抗性基因，其转移受到λ（434）cI相关阻遏物SetR的抑制。在这里，我们确定了不同的启动子之间的s086和setR驱动器的调节SXT转移的表达。一个转录本编码转移激活因子setC和setD。第二个转录本编码SetR，并且像lambda的cl转录本一样，是无前导的。SetR绑定到四个运营商之间的setR和s086，这些网站的位置和相对亲和力建议一个模型的调节SXT转移。
Transfer of SXT, a Vibrio cholerae-derived integrating conjugative element that encodes multiple antibiotic resistance genes, is repressed by SetR, a lambda(434) cI-related repressor. Here we identify divergent promoters between s086 and setR that drive expression of the regulators of SXT transfer. One transcript encodes the activators of transfer, setC and setD. The second transcript codes for SetR and, like the cl transcript of lambda, is leaderless. SetR binds to four operators located between setR and s086; the locations and relative affinities of these sites suggest a model for regulation of SXT transfer.