Unusually high frequency MHC class I alleles in Mauritian origin Cynomolgus macaques
Unusually high frequency MHC class I alleles in Mauritian origin Cynomolgus macaques
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DOI：
10.4049/jimmunol.175.8.5230
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发表时间：
2005-10-15
影响因子：
4.4
通讯作者：
O'Connor, DH
中科院分区：
文献类型：
--
作者：
Krebs, KC;Jin, ZY;O'Connor, DH
印度原产猕猴的严重短缺严重阻碍了艾滋病毒/艾滋病的研究。细胞免疫反应的研究特别困难，因为只有一小部分动物具有MHC I类(MHC I)等位基因，这些等位基因具有明确的肽结合特异性。为了扩大适合细胞免疫研究的非人类灵长类动物库，我们在中国猕猴、越南猕猴和毛里求斯猕猴中定义了66个MHC I等位基因。大多数MHC I等位基因只在同一地理来源的动物身上发现，这表明来自不同来源的食蟹猴在细胞免疫研究中不能互换。来自毛里求斯的动物可能特别有价值，因为这些食蟹猴中有50%共享MHC I类等位基因组合Mafa-B*430101、Mafa-11*440101和Mafa-B*460101。毛里求斯食蟹猴MHC I等位基因共享的增加可能会极大地减少研究非人类灵长类动物细胞免疫反应所需的动物总数，同时减少艾滋病毒/艾滋病研究中遗传异质性的混杂影响。
Acute shortages of Indian origin Rhesus macaques significantly hinder HIV/AIDS research. Cellular immune responses are particularly difficult to study because only a subset of animals possess MHC class I (MHC I) alleles with defined peptide-binding specificities. To expand the pool of nonhuman primates suitable for studies of cellular immunity, we defined 66 MHC I alleles in Cynomolgus macaques (Macaca fascicularis) of Chinese, Vietnamese, and Mauritian origin. Most MHC I alleles were found only in animals from a single geographic origin, suggesting that Cynomolgus macaques from different origins are not interchangeable in studies of cellular immunity. Animals from Mauritius may be particularly valuable because >50% of these Cynomolgus macaques share the MHC class I allele combination Mafa-B*430101, Mafa-11*440101, and Mafa-B*460101. The increased MHC I allele sharing of Mauritian origin Cynomolgus macaques may dramatically reduce the overall number of animals needed to study cellular immune responses in nonhuman primates while simultaneously reducing the confounding effects of genetic heterogeneity in HIV/AIDS research.