Rolling circle amplification promoted magneto-controlled photoelectrochemical biosensor for organophosphorus pesticides based on dissolution of core-shell MnO2 nanoflower@CdS mediated by butyrylcholinesterase
Rolling circle amplification promoted magneto-controlled photoelectrochemical biosensor for organophosphorus pesticides based on dissolution of core-shell MnO2 nanoflower@CdS mediated by butyrylcholinesterase
复制标题
滚环放大促进基于丁酰胆碱酯酶介导的核壳MnO2纳米花@CdS溶解的有机磷农药磁控光电化学生物传感器
DOI：
10.1007/s00604-020-04434-0
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发表时间：
2020-07-16
影响因子：
5.7
通讯作者：
Tang, Dianping
中科院分区：
文献类型：
--
作者：
Tang, Juan;Li, Jingjing;Tang, Dianping
利用硫代胆碱（TCh）对核壳MnO2nanoflower@CdS （MnO2NF@CdS）的溶解作用，设计了一种光电化学（PEC）适体感应平台，用于有机磷农药的灵敏检测。TCH是由丁基胆碱酯酶-乙酰硫代胆碱系统产生的，伴随着靶触发滚动圈扩增（RCA）。合成了具有优异PEC性能的核壳材料MnO2NF@CdS，并将其用作光传感平台。在具有相应适配体的功能化磁探针上检测目标。引入马拉硫磷后，适体从磁珠上分离，而捕获的DNA （cDNA，带引物片段）留在磁珠上。cDNA中的引物片段可以触发RCA反应，形成长单链DNA （ssDNA）。此外，通过与S2-Au-BChE探针的杂交，在ssDNA长链上组装了大量的丁基胆碱酯酶（BChE）。随后，由BChE水解ATCh生成的TCh可以将MnO2NF（核心）还原为Mn2+，并从平台电极释放CdS纳米粒子（壳），显著增强了PEC信号。在最佳条件下，该传感器对马拉硫磷具有较高的灵敏度，检出限为0.68 pg mL-1。同时，该方法具有良好的特异性、重复性和稳定性。重要的是，该传感平台为农药检测提供了新的概念。基于丁基胆碱酯酶-乙酰硫代胆碱体系生成的硫代胆碱（TCh）对核壳MnO2nanoflower@CdS （MnO2NF@CdS）的溶解，结合靶触发滚动环扩增（RCA），设计了一种光电化学（PEC）适体感应平台，用于有机磷农药的灵敏检测。
A photoelectrochemical (PEC) aptasensing platform is devised for sensitive detection of an organophosphorus pesticide based on dissolution of core-shell MnO2nanoflower@CdS (MnO2NF@CdS) by thiocholine (TCh). TCH is produced from the butyrylcholinesterase-acetylthiocholine system, accompanied by target-triggered rolling circle amplification (RCA). The core-shell MnO2NF@CdS with excellent PEC performance was synthesized and employed as a photo-sensing platform. The target was detected on a functionalized magnetic probe with the corresponding aptamer. Upon malathion introduction, the aptamer was detached from the magnetic beads, while capture DNA (cDNA, with primer fragment) remained on the beads. The primer fragment in cDNA can trigger the RCA reaction to form a long single-stranded DNA (ssDNA). Furthermore, a large number of butyrylcholinesterase (BChE) were assembled on the long ssDNA strands through the hybridization with the S2-Au-BChE probe. Thereafter, TCh generated from hydrolysis of ATCh by BChE can reduce MnO2NF (core) to Mn2+and release the CdS nanoparticles (shell) from the platform electrode, significantly enhancing the PEC signal. Under optimal conditions, the proposed aptasensor exhibited high sensitivity for malathion with a low detection limit of 0.68 pg mL-1. Meanwhile, it also presents outstanding specificity, reproducibility, and stability. Importantly, the sensing platform provides a new concept for detection of pesticide. Graphical abstract Herein, this work devised a photoelectrochemical (PEC) aptasensing platform for sensitive detection of organophosphorus pesticide based on dissolution of core-shell MnO2nanoflower@CdS (MnO2NF@CdS) by the as-produced thiocholine (TCh) from the butyrylcholinesterase-acetylthiocholine system, accompanying with the target-triggered rolling circle amplification (RCA).