Sample concentration using optical chromatography
Sample concentration using optical chromatography
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DOI：
10.1364/oe.15.002724
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发表时间：
2007-03-05
期刊：
影响因子：
3.8
通讯作者：
Leski, Tomasz A.
中科院分区：
文献类型：
--
作者：
Hart, Sean J.;Terray, Alex;Leski, Tomasz A.
光学色谱是一种利用光学力和流体力之间的平衡来分离颗粒的技术。当流体流中的微小颗粒遇到沿相反方向传播的激光束时，它们会沿着光束轴向被捕获。然后，它们被光学地从激光焦点推向上游，停留在粒子上的光学力和流体力平衡的点上。由于光学力和流体力对颗粒的物理和化学性质的差异敏感，因此粗分离和精细分离都是可能的。我们描述了如何将光学色谱光束引导到定制的流动环境中，作为光学过滤器进行胶体和生物样品的浓缩/生物富集。在这项工作中，所证明的浓缩生物战剂炭疽杆菌孢子的能力可能会对生物防御领域产生重大影响。这些技术的应用以及流体和光学环境的进一步设计将允许对更多微观颗粒和生物悬浮液进行更具体的识别、浓缩和分离。 (c) 2007 年美国光学学会。
Optical chromatography is a technique for the separation of particles that capitalizes on the balance between optic and fluidic forces. When microscopic particles in a fluid flow encounter a laser beam propagating in the opposite direction, they are trapped axially along the beam. They are then optically pushed upstream from the laser focal point to rest at a point where the optic and fluidic forces on the particle balance. Because optical and fluid forces are sensitive to differences in the physical and chemical properties of a particle, both coarse and fine separations are possible. We describe how an optical chromatography beam directed into a tailored flow environment, has been adapted to operate as an optical filter for the concentration / bioenrichment of colloidal and biological samples. In this work, the demonstrated ability to concentrate spores of the biowarfare agent, Bacillus anthracis, may have significant impact in the biodefense arena. Application of these techniques and further design of fluidic and optical environments will allow for more specific identification, concentration and separation of many more microscopic particle and biological suspensions. (c) 2007 Optical Society of America.