Whole-genome resequencing of wild and domestic sheep identifies genes associated with morphological and agronomic traits
Whole-genome resequencing of wild and domestic sheep identifies genes associated with morphological and agronomic traits
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DOI：
10.1038/s41467-020-16485-1
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发表时间：
2020-06-04
影响因子：
16.6
通讯作者：
Li, Meng-Hua
中科院分区：
文献类型：
--
作者：
Li, Xin;Yang, Ji;Li, Meng-Hua
了解绵羊（Ovis aries）表型变异背后的遗传变化可能有助于我们进一步改进。在这里，我们报告了248只羊的深度重测序，包括野生祖先（O。orientalis）、地方品种和改良品种。我们探索了绵羊的变异组和选择特征。我们检测到的基因组区域窝藏与不同的形态和农艺性状，这可能是过去和潜在的未来目标的驯化，育种和选择相关的基因。此外，我们发现在一组合理的候选基因和显着差异，其等位基因频率分布在不同品种的非同义突变。我们通过转录组、RT-PCR、qPCR和Western印迹分析确定PDGFD可能是绵羊尾部脂肪沉积的致病基因。我们的研究结果为绵羊的人口统计学历史提供了见解，并为未来的遗传研究和改善绵羊和其他家畜的基因组辅助育种提供了宝贵的基因组资源。家畜的遗传资源对于了解它们的人口统计学和过去的选择是有价值的，并且可以为未来的育种计划和农业改进提供信息。在这里，作者对248只野生和家养绵羊进行了深度重测序，并提供了相关数据和分析。
Understanding the genetic changes underlying phenotypic variation in sheep (Ovis aries) may facilitate our efforts towards further improvement. Here, we report the deep resequencing of 248 sheep including the wild ancestor (O. orientalis), landraces, and improved breeds. We explored the sheep variome and selection signatures. We detected genomic regions harboring genes associated with distinct morphological and agronomic traits, which may be past and potential future targets of domestication, breeding, and selection. Furthermore, we found non-synonymous mutations in a set of plausible candidate genes and significant differences in their allele frequency distributions across breeds. We identified PDGFD as a likely causal gene for fat deposition in the tails of sheep through transcriptome, RT-PCR, qPCR, and Western blot analyses. Our results provide insights into the demographic history of sheep and a valuable genomic resource for future genetic studies and improved genome-assisted breeding of sheep and other domestic animals. Genetic resources for livestock are valuable for understanding their demography and past selection, and may inform future breeding programs and agricultural improvement. Here, the authors present deep resequencing of 248 wild and domestic sheep, with associated data and analysis.