INHIBITORY EFFECT OF METHYLMERCURY ON MIGRATION AND TUBE FORMATION BY CULTURED HUMAN VASCULAR ENDOTHELIAL-CELLS
INHIBITORY EFFECT OF METHYLMERCURY ON MIGRATION AND TUBE FORMATION BY CULTURED HUMAN VASCULAR ENDOTHELIAL-CELLS
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DOI：
10.1007/s002040050184
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发表时间：
1995-05-01
影响因子：
6.1
通讯作者：
TADA, M
中科院分区：
文献类型：
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作者：
KISHIMOTO, T;OGURI, T;TADA, M
定量分析甲基汞（MeHg）对培养的人脐静脉内皮细胞（HUVEC）的运动和管形成的影响。测量内皮细胞生长距单层刮擦边缘的距离。 MeHg (1.0 - 5.0 muM) 治疗以剂量依赖性方式抑制 HUVEC 生长。通过在凝胶基底膜基质（Matrigel）上培养细胞来研究管形成。用 0.1-5.0 muM MeHg 处理 HUVEC 24 小时，抑制管形成，呈剂量依赖性。这些结果表明，HUVEC 的迁移和管形成易受 MeHg 细胞毒性的影响，并且 MeHg 可能损害内皮细胞功能，这可能参与动脉硬化的发病机制。
The effect of methylmercury chloride (MeHg) on mi,oration and tube formation by cultured human umbilical vein endothelial cells (HUVECs) was quantitatively analyzed. The distance of endothelial cell outgrowth from the scraped edge of a monolayer was measured. HUVEC outgrowth was inhibited by MeHg (1.0 - 5.0 mu M) treatment in a dose-dependent manner. Tube formation was studied by culturing the cells on gelled basement membrane matrix (Matrigel). Treatment of HUVECs with 0.1-5.0 mu M MeHg for 24 h inhibited tube formation dose-dependently. These results suggest that migration and tube formation by HUVECs are susceptible to MeHg cytotoxicity, and that MeHg could be injurious to endothelial cell function, which may be involved in the pathogenesis of arteriosclerosis.