A rapid enzyme immunofiltration technique using monoclonal antibodies to serotype herpes simplex virus.
A rapid enzyme immunofiltration technique using monoclonal antibodies to serotype herpes simplex virus.
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使用单克隆抗体对单纯疱疹病毒进行血清分型的快速酶免疫过滤技术。
DOI：
10.1002/jmv.1890090408
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发表时间：
1982
影响因子：
12.7
通讯作者：
Oxman,MN
中科院分区：
文献类型：
--
作者：
Richman,DD;Cleveland,PH;Oxman,MN
描述了使用单克隆抗体对单纯疱疹病毒进行血清分型的快速酶免疫过滤技术。它只需要显示病毒细胞病理学的 Gngle 管培养物，并且可以在一次测定中容纳多个样本。单克隆抗体具有绝对特异性，并且使用辣根过氧化物酶缀合的葡萄球菌蛋白 A 或抗球蛋白可以轻松直观地解读 2-3 小时测定后的结果。尽管偶尔有 HSV 野生株可能无法被单克隆抗体识别，但迄今为止在 500 多个临床分离株中尚未观察到这一潜在问题，所有这些都得出了明确的结果。
A rapid enzyme immunofiltration technique using monoclonal antibodies to serotype herpes simplex virus is described. It requires only a Gngle tube culture showing viral cytopathology and can accommodate multiple specimens in a single assay. The monoclonal antibodies confer absolute specificity and the use of horseradish peroxidase‐conjugated staphylococcal protein A or antiglobulin permits easy visual interpretation of the results following the 2‐3‐hour assay. Although it is possible that an occasional wild strain of HSV might escape recognition by monoclonal antibody, this potential problem has not been observed among the more than 500 clinical isolates tested to date, all of which have yielded an unequivocal result.