Localization of heat shock protein in osteoarthritic cartilage.
Localization of heat shock protein in osteoarthritic cartilage.
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热休克蛋白在骨关节炎软骨中的定位。
DOI：
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发表时间：
1997
影响因子：
2.1
通讯作者：
Yasusuke Hirasawa
中科院分区：
文献类型：
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作者：
Kenji Takahashi;Toshikazu Kubo;Y. Arai;Jiro Imanishi;Mitsuhiro Kawata;Yasusuke Hirasawa
在骨关节炎（OA）软骨细胞中，70 kD热休克蛋白（HSP 70）的表达增强应力。HSP 70水平也与人类OA的严重程度有关。在本研究中，OA软骨标本来自14名手术治疗的女性患者。根据Mankin的组织学/组织化学分级评分评估其疾病严重程度。用免疫组化法检测HSP 70阳性细胞的比例。随着OA的加重，HSP 70阳性细胞的比例在较深的区域增加，并且在级别和比例之间呈正相关。原位杂交检测到HSP 70 mRNA阳性细胞的分布与免疫组化检测到的阳性细胞分布相似。推测HSP 70在细胞应激保护中起作用，并且与HSP 70表达增强相关的机械和生物学因素对OA进展具有相当大的影响。
In osteoarthritic (OA) chondrocytes, expression of 70 kD heat shock protein (HSP70) is enhanced under stress. The HSP70 level is also related to OA severity in humans. In the present study, specimens of OA cartilage were obtained from 14 surgically treated female patients. Their disease severity was evaluated according to Mankin's histological/histochemical grading scores. The ratio of HSP70-positive cells was examined by using immunohistochemistry. As OA became more severe, the ratio of HSP70-positive cells increased in deeper areas from the surface, and there was a positive correlation between the grade and the ratio. Distribution of cells expressing HSP70 mRNA detected by in situ hybridization was similar to the distribution of positive cells in the immunohistochemistry. It was presumed that HSP70 plays a role in cell protection from stress, and that mechanical and biological factors which are related to the enhancement of HSP70 expression, have a considerable influence on OA progressions.
骨关节炎患者软骨细胞中应激诱导的蛋白质。
影响因子：
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作者：
Kubo,T;Towle,CA;Mankin,HJ;Treadwell,BV
通讯作者：
Treadwell,BV