Transcriptional responses of Saccharomyces cerevisiae to preferred and nonpreferred nitrogen sources in glucose-limited chemostat cultures
Transcriptional responses of Saccharomyces cerevisiae to preferred and nonpreferred nitrogen sources in glucose-limited chemostat cultures
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DOI：
10.1111/j.1567-1364.2007.00220.x
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发表时间：
2007-06-01
影响因子：
3.2
通讯作者：
Daran, Jean-Marc
中科院分区：
文献类型：
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作者：
Boer, Viktor M.;Tai, Siew Leng;Daran, Jean-Marc
对6种不同氮源的酿酒酵母好氧、葡萄糖限量恒化培养物进行了转录组分析。化学抑制剂的使用使得在固定的特定生长速度下分析依赖氮源的转录调控成为可能。对首选氮源(铵和天冬酰胺)和非首选氮源(亮氨酸、苯丙氨酸、蛋氨酸和脯氨酸)的选择进行了研究。对于每一种氮源，相对于其他五种氮源，不同的基因集被诱导或抑制。在这项研究中，总共确定了131份这样的“签名成绩单”。除了签名转录本外，还发现了对六种氮源中的两种或两种以上表现出转录协同反应的基因。例如，33个基因在亮氨酸、苯丙氨酸和蛋氨酸培养物中转录上调；这部分归因于共同的酶参与了这些氨基酸的异化。除了个别氮源引起的特定转录反应外，还监测了它们对全球调控机制的影响，如氮分解代谢抑制(NCR)。恒化器培养中NCR敏感基因的表达表明，铵和天冬酰胺是富含的氮源。根据这一标准，亮氨酸、脯氨酸和蛋氨酸是“差”的氮源，而苯丙氨酸表现出“中等”的NCR反应。
Aerobic, glucose-limited chemostat cultures of Saccharomyces cerevisiae grown with six different nitrogen sources were subjected to transcriptome analysis. The use of chemostats enabled an analysis of nitrogen-source-dependent transcriptional regulation at a fixed specific growth rate. A selection of preferred (ammonium and asparagine) and nonpreferred (leucine, phenylalanine, methionine and proline) nitrogen sources was investigated. For each nitrogen source, distinct sets of genes were induced or repressed relative to the other five nitrogen sources. In total, 131 such 'signature transcripts' were identified in this study. In addition to signature transcripts, genes were identified that showed a transcriptional coresponse to two or more of the six nitrogen sources. For example, 33 genes were transcriptionally upregulated in leucine-grown, phenylalanine-grown and methionine-grown cultures; this was partly attributed to the involvement of common enzymes in the dissimilation of these amino acids. In addition to specific transcriptional responses elicited by individual nitrogen sources, their impact on global regulatory mechanisms such as nitrogen catabolite repression (NCR) were monitored. NCR-sensitive gene expression in the chemostat cultures showed that ammonium and asparagine were 'rich' nitrogen sources. By this criterion, leucine, proline and methionine were 'poor' nitrogen sources, and phenylalanine showed an 'intermediate' NCR response.