Carboxylated Pocoa polysaccharides inhibited oxidative damage and inflammation of HK-2 cells induced by calcium oxalate nanoparticles.
Carboxylated Pocoa polysaccharides inhibited oxidative damage and inflammation of HK-2 cells induced by calcium oxalate nanoparticles.
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DOI：
10.1016/j.biopha.2023.115865
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发表时间：
2023-11
期刊：
影响因子：
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通讯作者：
Peng Xiong;Yu-Yun Zheng;J. Ouyang
中科院分区：
文献类型：
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作者：
Peng Xiong;Yu-Yun Zheng;J. Ouyang
研究了不同羧基（-COOH）含量的中药茯苓多糖（pcp）对肾上皮细胞氧化损伤和炎症反应的抑制作用，以及多糖中-COOH含量的影响。采用纳米草酸钙晶体（nanoCOM）建立HK-2细胞损伤模型，然后采用-COOH含量分别为2.56% （PCP0）、7.48% （PCP1）、12.07% （PCP2）和17.18% （PCP3）的pcp对细胞进行保护。pcp能抑制nanoCOM对HK-2细胞的损伤，提高细胞活力，恢复细胞骨架和形态，改善溶酶体完整性。ppps可以降低细胞对nanoCOM的氧化应激反应，抑制mPTP的开放和细胞坏死性凋亡，降低细胞内Ca2+离子水平、ATP和MDA的产生，增加SOD的表达。pcp还能降低氧化损伤引起的细胞炎症反应，降低一氧化氮（NO）、炎症因子TNF-α、IL-6、IL-1β和MCP-1的表达以及炎性小体NLRP3的含量。经过保护后，pcp可以抑制细胞对纳米com晶体的内吞作用。随着pcp中-COOH含量的增加，其抑制nanoCOM细胞损伤、减轻氧化应激、减轻炎症反应、抑制晶体内吞的能力增强，即-COOH含量最高的PCP3表现出最好的生物活性。抑制细胞损伤和炎症，减少细胞对晶体的大量内吞，有利于抑制肾结石的形成。
The inhibitory effects of Chinese medicine Pocoa (PCPs) with different carboxyl group (-COOH) contents on oxidative damage and inflammatory response of renal epithelial cells and the influence of -COOH content in polysaccharides were investigated. HK-2 cell damage model was established by nanocalcium oxalate crystals (nanoCOM), and then PCPs with -COOH contents of 2.56% (PCP0), 7.48% (PCP1), 12.07% (PCP2), and 17.18% (PCP3) were used to protect the cells. PCPs could inhibit the damage of nanoCOM to HK-2 cells, increase cell viability, restore cytoskeleton and morphology, and improve lysosomal integrity. PCPs can reduce the oxidative stress response of nanoCOM to cells, inhibit the opening of mPTP and cell necrotic apoptosis, reduce the level of Ca2+ions in cells, the production of ATP and MDA, and increase SOD expression. PCPs can also reduce the cellular inflammatory response caused by oxidative damage, and reduce the expression of nitric oxide (NO), inflammatory factors TNF-α, IL-6, IL-1β and MCP-1, as well as the content of inflammasome NLRP3. After protection, PCPs can inhibit the endocytosis of nanoCOM crystals by cells. With the increase in -COOH content in PCPs, its ability to inhibit nanoCOM cell damage, reduce oxidative stress, reduce inflammatory response, and inhibit crystal endocytosis increases, that is, PCP3 with the highest -COOH content, shows the best biological activity. Inhibiting cell damage and inflammation and reducing a large amount of endocytosis of crystals by cells are beneficial to inhibit the formation of kidney stones.