-Fructofuranosidases from roots of dandelion (Taraxacum officinale Weber).
-Fructofuranosidases from roots of dandelion (Taraxacum officinale Weber).
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-来自蒲公英（Taraxacum officinale Weber）根的呋喃果糖苷酶。
DOI：
10.1042/bj1260569
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发表时间：
1972
影响因子：
4.1
通讯作者：
A. Deacon
中科院分区：
文献类型：
--
作者：
P. Rutherford;A. Deacon
1.用DEAE-纤维素柱从蒲公英(Taraxacum Office Inale Weber)根部提取的可溶性蛋白中分离得到3种β-呋喃果糖苷酶。2.一种作用于蔗糖的酶为转化酶，其K(M)为2.00×10~(-2)m，最适pH为7.5。3.另外两种酶是水解酶(A和B)，作用于菊糖系列低聚糖[通式为葡萄糖-果糖-(果糖)(N)]。它们的最适pH为4.0，K(M)为1.54×10~(-2)m，但它们在DEAE-纤维素上的层析行为不同。这两种水解酶都不受蔗糖的抑制。4.讨论了这三种水解酶的生理作用。
1. Three beta-fructofuranosidases were separated by chromatography on a DEAE-cellulose column from the soluble protein extracted from dandelion (Taraxacum officinale Weber) roots. 2. One enzyme, which acted on sucrose, was characterized as an invertase, with a K(m) of 2.00x10(-2)m and pH optimum of 7.5. 3. The other two enzymes are hydrolases (A and B), which act on the inulin series of oligosaccharides [general formula glucose-fructose-(fructose)(n)]. They both have a pH optimum of 4.0 and K(m) of 1.54x10(-2)m but differ in their chromatographic behaviour on DEAE-cellulose. Neither of the hydrolases is inhibited by sucrose. 4. The physiological role of these three hydrolytic enzymes is discussed.