EXPRESSION OF MESSENGER-RNA FOR INTERLEUKIN-5 IN MUCOSAL BRONCHIAL BIOPSIES FROM ASTHMA
EXPRESSION OF MESSENGER-RNA FOR INTERLEUKIN-5 IN MUCOSAL BRONCHIAL BIOPSIES FROM ASTHMA
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DOI：
10.1172/jci115166
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发表时间：
1991-05-01
影响因子：
15.9
通讯作者：
KAY, AB
中科院分区：
文献类型：
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作者：
HAMID, Q;AZZAWI, M;KAY, AB
我们尝试使用原位杂交技术来鉴定哮喘患者和对照组的支气管内粘膜活检中的 IL-5 mRNA。 从 10 名哮喘患者和 9 名非过敏性正常对照中获得支气管活检。 从 IL-5 cDNA 制备放射性标记的 cRNA 探针，并与透化切片杂交。 在进行放射自显影之前，将它们进行彻底清洗。 来自患有高 IgE 综合征的患者的产生 IL-5 的 T 细胞克隆被用作阳性对照。 作为阴性对照，切片也用“有义”IL-5 探针处理。 10 名哮喘受试者中有 6 名的支气管粘膜内存在 IL-5 mRNA 的特异性杂交信号。 表现出杂交信号的细胞位于上皮基底膜下方。 相反，对照组没有杂交。 用有义探针未观察到杂交。根据症状和肺功能评估，六名 IL-5 mRNA 阳性哮喘患者的病情往往比阴性哮喘患者更严重，并且通过分泌 (EG2+) 嗜酸性粒细胞和活化 (CD25+) T 淋巴细胞显示支气管粘膜浸润程度显着增加。 在IL-5 mRNA阳性的受试者中，IL-5 mRNA的表达与CD25+和EG2+细胞的数量以及嗜酸性粒细胞总数之间存在相关性。这项研究为哮喘患者支气管粘膜中IL-5 mRNA的细胞定位提供了证据，并支持了这种细胞因子在支气管哮喘中调节嗜酸性粒细胞功能的概念。
We have attempted to identify mRNA for IL-5 in endobronchial mucosal biopsies from asthmatics and controls, using the technique of in situ hybridization. Bronchial biopsies were obtained from 10 asthmatics and 9 nonatopic normal controls. A radio-labeled cRNA probe was prepared from an IL-5 cDNA and hybridized to permeabilized sections. These were washed extensively before processing for autoradiography. An IL-5-producing T cell clone derived from a patient with the hyper-IgE syndrome was used as a positive control. As a negative control, sections were also treated with a "sense" IL-5 probe. Specific hybridization signals for IL-5 mRNA were demonstrated within the bronchial mucosa in 6 out of the 10 asthmatic subjects. Cells exhibiting hybridization signals were located beneath the epithelial basement membrane. In contrast, there was no hybridization in the control group. No hybridization was observed with the sense probe.The six IL-5 mRNA-positive asthmatics tended to have more severe disease than the negative asthmatics, as assessed by symptoms and lung function, and showed a significant increase in the degree of infiltration of the bronchial mucosa by secreting (EG2+) eosinophils and activated (CD25+) T lymphocytes. Within the subjects who showed positive IL-5 mRNA, there was a correlation between IL-5 mRNA expression and the number of CD25+ and EG2+ cells and total eosinophil count.This study provides evidence for the cellular localization of IL-5 mRNA in the bronchial mucosa of asthmatics and supports the concept that this cytokine regulates eosinophil function in bronchial asthma.