A method for the determination of fructose using a single enzyme : production and properties of fructose dehydrogenase from Gluconobacter industrius
A method for the determination of fructose using a single enzyme : production and properties of fructose dehydrogenase from Gluconobacter industrius
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单一酶测定果糖的方法：工业葡糖杆菌果糖脱氢酶的产生和性质
DOI：
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发表时间：
1994
影响因子：
2.8
通讯作者：
A. R. Sampietro
中科院分区：
文献类型：
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作者：
F. Prado;A. R. Sampietro
本文报道了一种从工业葡萄糖酵母中生产、溶解和纯化果糖脱氢酶的简便方法。G. Industrius在含有蔗糖的液体培养基中生长。使用Trlton X-100、毛地黄皂苷和KCl溶解酶。溶解的酶在SDS/PAGE上显示一条蛋白质带，等电聚焦显示pI为4.55。纯化的果糖脱氢酶为天然形式，全酶的Mr为130000，每个亚基的Mr为63000
A simple method for the production, solubilization and purification of fructose dehydrogenase from Gluconobacter industrius has been developed. G. industrius was grown in a liquid medium with sucrose. The enzyme was solubilized using Trlton X-100, digitonin and KCl. The solubilized enzyme shows one protein band on SDS/PAGE and on isoelectric focusing indicates a pI of 4.55. The purified fructose dehydrogenase is a differ in the native form, with an M r of 130 000 for the whole enzyme and 63 000 for each subunit respectively