Detection of ketosis in vitreous at autopsy after embalming.
Detection of ketosis in vitreous at autopsy after embalming.
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防腐处理后尸检时检测玻璃体酮症。
DOI：
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发表时间：
2002
影响因子：
1.6
通讯作者：
C. Andrew Robinson
中科院分区：
文献类型：
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作者：
Jon R. Steinhauer;Andrea L. Volk;R. Hardy;R. Konrad;Thomas M. Daly;C. Andrew Robinson
酮症发生在酮症酸中毒或营养不良。当怀疑与死亡有关时，在尸检时分析酮症可能很重要。我们遇到了一个案例，一个已故的养老院居民被怀疑饥饿，尸体在尸检前已经防腐。气相色谱法（GC）无法将丙酮与甲醛（防腐液的一种成分）分离。Acetest是一种简单的测试，可以检测体液中的丙酮和乙酰乙酸。我们用GC对玻璃体进行了Acetest验证。Acetest和GC是一致的，除了在非常低水平的丙酮或乙酰乙酸。Acetest对玻璃体中乙酰乙酸的灵敏度为10 mg/dL，与早期饥饿一致。防腐液的显著干扰没有发生。在所述病例中，Acetest为阴性。Acetest是一个简单而有用的测试，用于检测尸体防腐处理中的酮症。
Ketosis occurs in ketoacidosis or malnourishment. When either is suspected in relation to a death, it may be important to analyze for ketosis at autopsy. We encountered a case where starvation was suspected in a deceased nursing home resident, where the body had been embalmed prior to autopsy. Gas chromatography (GC) was unable to separate acetone from formaldehyde, a component of embalming fluid. The Acetest is a simple test that can detect acetone and acetoacetate in body fluids. We validated the Acetest with GC on vitreous. The Acetest and GC were consistent except at very low levels of acetone or acetoacetate. The sensitivity of the Acetest for acetoacetate in vitreous was 10 mg/dL, consistent with early starvation. Significant interference from embalming fluid did not occur. The Acetest was negative in the described case. The Acetest is a simple and useful test for the detection of ketosis in embalmed autopsies.