gamma-Aminobutyric acid and benzodiazepine receptors: copurification and characterization.
gamma-Aminobutyric acid and benzodiazepine receptors: copurification and characterization.
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γ-氨基丁酸和苯二氮卓受体：共纯化和表征。
DOI：
10.1073/pnas.78.3.1939
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发表时间：
1981
影响因子：
11.1
通讯作者：
Snyder,SH
中科院分区：
文献类型：
--
作者：
Gavish,M;Snyder,SH
γ-氨基丁酸（GABA）和苯并二氮杂受体已经通过诸如凝胶过滤、离子交换、凝集素和亲和色谱法的程序溶解和纯化。所有这些程序都能以相似的程度增强每种受体的比活性。[3 H]蝇蕈醇和[3 H]氟硝西泮结合位点的药物特异性在通过亲和色谱法进行广泛纯化后与膜结合和最初溶解的受体相比是相同的。苯二氮卓类结合的GABA和氯刺激保留在纯受体中。纯化的受体经紫外线照射后，用[3 H]氟硝西泮共价标记两条带。GABA，苯并二氮卓类，和氯离子识别位点的持久性协会广泛纯化后，表明它们可能是一个单一的大分子复合物的一部分。
gamma-Aminobutyric acid (GABA) and benzodiazepine receptors have been solubilized and purified by procedures such as gel filtration, ion-exchange, lectin, and affinity chromatographies. All of these procedures enhance the specific activity of each receptor to a similar extent. The drug specificities of [3H]muscimol and [3H]flunitrazepam binding sites are the same after extensive purification by affinity chromatography compared to the membrane bound and initially solubilized receptors. GABA and chloride stimulation of benzodiazepine binding is retained in pure receptors. Two bands are covalently labeled with [3H]flunitrazepam after ultraviolet irradiation of the purified receptor. The persistent association of GABA, benzodiazepine, and chloride recognition sites after extensive purification suggests that they may be part of a single macromolecular complex.