Oestrogen receptor in the baboon endometrium: cytosolic receptor, detection, characterization and variation of its concentration during the menstrual cycle.
Oestrogen receptor in the baboon endometrium: cytosolic receptor, detection, characterization and variation of its concentration during the menstrual cycle.
复制标题
狒狒子宫内膜中的雌激素受体：胞质受体、检测、表征及其在月经周期期间浓度的变化。
作者：
D. Martel;C. Malet;C. Olmedo;M. Monier;P. Dubouch;A. Psychoyos
从狒狒子宫内膜中检测到一种细胞质雌激素受体，并对其进行了部分表征。雌二醇的表观解离常数为1.5 × 10(-10) ~ 4 × 10(-10) mol/l。与与受体结合的[3H]雌二醇竞争的类固醇是雌二醇和乙炔雌二醇大于雌三醇大于雌酮；黄体酮、睾酮和皮质酮不是竞争对手。无论在增殖期还是分泌期取子宫内膜样品，蔗糖密度梯度离心时，[3H]雌二醇受体复合物都以3-3.5S的峰值迁移。在排卵期的样品中观察到7S峰。采用[3H]雌二醇交换技术检测月经周期中受体浓度的变化。在卵泡期早期较高的浓度在卵巢雌激素的排卵高峰前急剧下降。它在分泌初期保持在一个基础水平，然后在周期的最后几天上升到与周期开始时测量的浓度相同。
A cytosolic oestrogen receptor from baboon endometria was detected and partially characterized. The apparent dissociation constant for oestradiol was 1.5 x 10(-10)--4 x 10(-10) mol/l. Steroids that competed with the [3H]oestradiol binding to the receptor were oestradiol and ethynyloestradiol greater than oestriol greater than oestrone; progesterone, testosterone and corticosterone were not competitors. The [3H]oestradiol-receptor complexes migrated as a 3-3.5S peak during sucrose density-gradient centrifugation when endometrial samples were taken during either the proliferative or the secretory phase. A 7S peak was observed for samples taken at the period of ovulation. A [3H]oestradiol exchange technique was used to detect changes in the receptor concentration during the menstrual cycle. This concentration which was high during the early follicular phase fell sharply before the ovulatory peak of ovarian oestrogens. It remained at a base level during the early secretory phase and then rose during the last days of the cycle to the same concentration as that measured at the beginning of the cycle.