MYC Copy Number Gains are Associated with Poor Outcome in Penile Squamous Cell Carcinoma
MYC Copy Number Gains are Associated with Poor Outcome in Penile Squamous Cell Carcinoma
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DOI：
10.1016/j.juro.2012.07.003
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发表时间：
2012-11-01
影响因子：
6.6
通讯作者：
Toll, Agusti
中科院分区：
文献类型：
--
作者：
Masferrer, Emili;Ferrandiz-Pulido, Carla;Toll, Agusti
目的：研究MYC基因在阴茎鳞状细胞癌发生发展过程中的数目畸变和蛋白表达。我们将这些发现与临床病理参数和HPV infection.Materials和方法：我们评估了79例阴茎鳞状细胞癌，其中原位癌11例，浸润癌68例。用荧光原位杂交技术检测MYC细胞遗传学特征。结果：11例阴茎原位癌中4例（36%）和68例阴茎浸润性鳞癌中50例（73%）检出MYC阳性。MYC增加与肿瘤进展和不良结局之间存在显著相关性（p < 0.05）。79例阴茎鳞状细胞癌中32例（39%）检出HPV DNA。高危型16是最常见的类型。MYC数目畸变与HPV状态无关。HPV和MYC蛋白过度表达之间存在显著相关性。在HPV阴性的病例中，MYC的增加与MYC的过度表达相关。结论：在阴茎鳞状细胞癌从原位样本到转移的进展过程中，MYC的增加逐渐增加。MYC增加是预后不良的独立因素。这些发现与HPV感染无关。在HPV感染的样本中MYC表达增加，可能反映了MYC的直接激活。
Purpose: We determined MYC gene numerical aberrations and protein expression at different stages of penile squamous cell carcinoma carcinogenesis. We correlated these findings with clinicopathological parameters and HPV infection.Materials and Methods: We evaluated 79 cases of penile squamous cell carcinoma, including 11 in situ and 68 invasive carcinomas. The MYC cytogenetic profile was evaluated by fluorescence in situ hybridization. HPV was detected by polymerase chain reaction amplification.Results: MYC gains were identified in 4 of 11 in situ carcinomas (36%) and 50 of 68 invasive penile squamous cell carcinomas (73%). A significant association between MYC gains, and tumor progression and poor outcome was demonstrated (p < 0.05). HPV DNA was detected in 32 of 79 penile squamous cell carcinomas (39%). High risk type 16 was the most prevalent type. MYC numerical aberrations did not correlate with HPV status. A significant association between HPV and MYC protein over expression was noted. In HPV negative cases MYC gains correlated with MYC over expression.Conclusions: MYC gains progressively increased during penile squamous cell carcinoma progression from in situ samples to metastases. MYC gains were an independent factor for poor prognosis. These findings were independent of HPV infection. MYC expression was increased in samples with HPV infection, probably reflecting direct activation of MYC.