Transcription factor E74A affects the ecdysone titer by regulating the expression of the EO gene in the silkworm, Bomby mori
Transcription factor E74A affects the ecdysone titer by regulating the expression of the EO gene in the silkworm, Bomby mori
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转录因子E74A通过调节家蚕EO基因的表达影响蜕皮激素滴度
DOI：
10.1016/j.bbagen.2016.11.017
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Zhang Z
中科院分区：
文献类型：
--
作者：
Sun W;Wang CF;Zhang Z
昆虫体内蜕皮激素脉冲的形成受其生物合成和降解的协同控制。以前的研究揭示了前胸腺(PG)活动的反馈调节，以影响激素的合成。然而，蜕皮激素降解的分子调控机制尚不清楚。在本研究中，我们发现在家蚕中编码激素代谢酶的蜕皮酮氧化酶(EO)基因也是由激素本身诱导的。此外，荧光素酶报告、染色质免疫沉淀和电泳迁移率改变分析表明，蜕皮激素诱导转录因子E74A可以与EO基因的顺式调控元件结合。然后，通过RNA干扰(RNAi)下调E74A的表达，降低EO基因的表达，并导致与对照相比更高的蜕皮激素滴度。因此，我们的结果证明了一种新的反馈调节降解(EO)途径，该途径由蜕皮激素本身通过转录因子E74A控制，扩展了关于决定蜕皮激素脉冲形成的调控系统的知识。
The formation of ecdysone pulse in insects is synergistically controlled by its biosynthesis and degradation. Previous studies have revealed the feedback regulation of the prothoracic gland (PG) activity to affect the hormone synthesis. However, the molecular regulatory mechanism of the ecdysone degradation is still unclear. In this study, we showed that ecdysone oxidase (EO) gene encoding a hormone metabolism enzyme was also induced by hormone itself in the domestic silkworm,Bombyx mori. Furthermore, luciferase reporter, chromatin immunoprecipitation and electrophoretic mobility shift assays showed that ecdysone inducible transcription factor E74A could bind to thecis-regulatory elements of theEOgene. Then, down-regulating the expression of theE74Aby RNA interference (RNAi) decreased the expression of theEOgene and caused a higher ecdysone titer compared with the control. Thus, our results demonstrated a new feedback regulation degradation (EO) pathway controlled by ecdysone itself through transcription factor E74A, expanding the knowledge about the regulatory system that determines the formation of ecdysone pulse.