In vivo release of neuronal histamine in the hypothalamus of rats measured by microdialysis
In vivo release of neuronal histamine in the hypothalamus of rats measured by microdialysis
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通过微透析测量大鼠下丘脑神经元组胺的体内释放
DOI：
10.1007/bf00168609
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
H. Wada
中科院分区：
文献类型：
--
作者：
T. Mochizuki;A. Yamatodani;K. Okakura;M. Takemura;N. Inagaki;H. Wada
本研究采用在体脑内微透析结合高效液相色谱荧光法，研究了麻醉大鼠下丘脑前区细胞外内源性组胺的水平。本方法测定的下丘脑前区内源性组胺释放的基础速率为0.09 ± 0.01 pmol/20 min。当通过透析膜注入100 mM K+或通过植入同侧结节乳头核的电极施加200 μ A的电刺激使下丘脑前区去极化时，组胺释放分别增加到基础水平的175%和188%。这些增加被完全抑制细胞外Ca 2+的去除。输注10−6 M河豚毒素或i. p.给予100 mg/kg α-氟甲基组氨酸后，组胺的基础释放也受到抑制。另一方面，在i. p.给药5 mg/kg硫代哌丁胺。这些结果清楚地表明，通过我们的方法测量的组胺的基础和诱发释放都是神经源性的。
SummaryUsing an in vivo intracerebral microdialysis method coupled with an HPLC-fluorometric method, we investigated the extracellular level of endogenous histamine in the anterior hypothalamic area of urethaneanaesthetized rats. The basal rate of release of endogenous histamine in the anterior hypothalamic area measured by this method was 0.09 + 0.01 pmol/20 min. When the anterior hypothalamic area was depolarized by infusion of 100 mM K+ through the dialysis membrane or electrical stimulation at 200 μ A was applied through an electrode implanted into the ipsilateral tuberomammillary nucleus, histamine release increased to 175% and 188%, respectively, of the basal level. These increases were completely suppressed by removal of extracellular Ca2+. The basal release of histamine was also suppressed after infusion of 10−6 M tetrodotoxin or i.p. administration of 100 mg/kg of α-fluoromethylhistidine. On the other hand, 3-fold increase in the basal release was observed after i. p. administration of 5 mg/kg thioperamide. These results clearly indicate that both the basal and evoked release of histamine measured by our method are of neuronal origin.
组胺作为神经调节剂。
影响因子：
13.9
作者：
Prell,GD;Green,JP
通讯作者：
Green,JP