Early Strides in NMR Dynamics Measurements.
Early Strides in NMR Dynamics Measurements.
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DOI：
10.1021/acs.biochem.1c00141
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发表时间：
2021-11-23
期刊：
影响因子：
2.9
通讯作者：
Dyson HJ
中科院分区：
文献类型：
--
作者：
Dyson HJ
利用核磁共振测量弛豫时间来研究蛋白质动力学是基于世纪中期的一系列研究，这些研究概述了理论和方法。然而，蛋白质NMR的复杂性使得这些简单的实验对于蛋白质的应用是不实际的。20世纪80年代出现的蛋白质同位素标记技术意味着脉冲序列现在可以应用于多维NMR实验，以实现局部弛豫时间的每个残基信息。最早的进展之一发表在1989年的《生物化学》上。刘易斯凯、丹尼斯托奇亚和Ad Bax的论文“15 N反向检测异源NMR光谱研究蛋白质的骨架动力学：应用于葡萄球菌核酸酶”，描述了一组即使在今天也进行了微小修改的脉冲序列。这篇论文，与其他来自其他实验室的有限数量，形成了蛋白质骨架动力学实验测定的基础。从这些测量中获得的生物学见解在过去30年中只增加了。有时候，推动一个领域发展的最佳方式，或许也是唯一的方式，是技术能力的进步，从而使新的视角得以实现。
The study of protein dynamics using the measurement of relaxation times by NMR was based on a set of studies in the mid-20th century that outlined theories and methods. However, the complexity of protein NMR was such that these simple experiments were not practical for application to proteins. The advent of techniques in the 1980s for isotopic labeling of proteins meant that pulse sequences could now be applied in multi-dimensional NMR experiments to enable per-residue information on the local relaxation times. One of the earliest advances was published in Biochemistry in 1989. The paper “Backbone dynamics of proteins as studied by 15N inverse detected heteronuclear NMR spectroscopy: application to staphylococcal nuclease” by Lewis Kay, Dennis Torchia and Ad Bax, delineated a set of pulse sequences that are used with minor modifications even today. This paper, with others from a limited number of other labs, forms the basis for the experimental determination of the backbone dynamics of proteins. The biological insights obtained from such measurements have only increased in the last 30 years. Sometimes the best and perhaps only way to advance a field is an advancement in the technical capabilities that allows new perspectives to be reached.
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影响因子：
15
作者：
LIPARI, G;SZABO, A
通讯作者：
SZABO, A
影响因子：
56.9
作者：
Boehr, David D.;McElheny, Dan;Wright, Peter E.
通讯作者：
Wright, Peter E.
影响因子：
2.9
作者：
TORCHIA, DA;SPARKS, SW;BAX, A
通讯作者：
BAX, A
影响因子：
3.4
作者：
LIPARI, G;SZABO, A
通讯作者：
SZABO, A
影响因子：
56.9
作者：
Eisenmesser, EZ;Bosco, DA;Kern, D
通讯作者：
Kern, D