Plasmon-Enhanced Fluorescence Biosensors: a Review.
Plasmon-Enhanced Fluorescence Biosensors: a Review.
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DOI：
10.1007/s11468-013-9660-5
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Dostalek J
中科院分区：
文献类型：
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作者：
Bauch M;Toma K;Toma M;Zhang Q;Dostalek J
金属薄膜和金属纳米粒子表面可以通过与传播或局域表面等离子体的耦合而对电磁场产生强烈的约束作用。这种相互作用与有机染料和量子点发射的荧光的场强和局域态密度的大幅度增强有关，这为提高激发速率、提高量子产额和控制荧光的远场角分布提供了手段。这种发射器通常用作检测化学和生物物种的分析中的标记。它们与表面等离子激元的相互作用使伴随分子结合事件的荧光信号(亮度)放大了几个数量级。等离子体增强荧光(PEF)，也称为金属增强荧光(MEF)，与用于在金属表面特定捕获目标分析物的界面结构相结合，是缩短检测时间和提高各种基于荧光的分析技术的灵敏度的一种有吸引力的方法。这篇综述介绍了脉冲电场的基本原理，阐述了利用传播和局域表面等离子体进行高效荧光信号放大的金属纳米结构设计的最新进展，并总结了生物传感器在检测与医学诊断和食品控制相关的痕量生物标志物、毒素和病原体方面的应用。
Surfaces of metallic films and metallic nanoparticles can strongly confine electromagnetic field through its coupling to propagating or localized surface plasmons. This interaction is associated with large enhancement of the field intensity and local optical density of states which provides means to increase excitation rate, raise quantum yield, and control far field angular distribution of fluorescence light emitted by organic dyes and quantum dots. Such emitters are commonly used as labels in assays for detection of chemical and biological species. Their interaction with surface plasmons allows amplifying fluorescence signal (brightness) that accompanies molecular binding events by several orders of magnitude. In conjunction with interfacial architectures for the specific capture of target analyte on a metallic surface, plasmon-enhanced fluorescence (PEF) that is also referred to as metal-enhanced fluorescence (MEF) represents an attractive method for shortening detection times and increasing sensitivity of various fluorescence-based analytical technologies. This review provides an introduction to fundamentals of PEF, illustrates current developments in design of metallic nanostructures for efficient fluorescence signal amplification that utilizes propagating and localized surface plasmons, and summarizes current implementations to biosensors for detection of trace amounts of biomarkers, toxins, and pathogens that are relevant to medical diagnostics and food control.