Oral Epithelial Cells are Activated via TRP Channels
Oral Epithelial Cells are Activated via TRP Channels
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DOI：
10.1177/0022034510385459
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发表时间：
2011-02
影响因子：
7.6
通讯作者：
Bing Wang;Atsushi Danjo;Atsushi Danjo;H. Kajiya;Koji Okabe;M. Kido
中科院分区：
文献类型：
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作者：
Bing Wang;Atsushi Danjo;Atsushi Danjo;H. Kajiya;Koji Okabe;M. Kido
瞬时受体电位（TRP）离子通道是感知各种环境刺激的关键因素。虽然口腔是各种食物的入口点，但TRP通道在口腔粘膜中的表达仍然未知。我们假设口腔上皮本身可能参与感知热，机械和化学条件。TRP通道的表达谱在颊、腭和舌上皮中表现出区域差异。用辣椒素、樟脑、4α-佛波醇-12，13-双癸酸酯（4α-PDD）、2-氨基乙氧基二苯基硼酸酯（2-APB）和薄荷醇刺激TRP通道后，口腔上皮细胞内[Ca ~（2+）]i升高。这些Ca 2+的增加似乎依赖于TRP通道，因为在加入TRPV通道拮抗剂钌红后观察到[Ca 2 +]i抑制。这些结果表明，口腔上皮细胞表达多种TRP通道，并可能与神经元一起在感觉活动中发挥功能作用。
Transient receptor potential (TRP) ion channels are critical contributors to the perception of various environmental stimuli. Although the oral cavity is the access point for various food types, the expression of TRP channels in oral mucosa remains unknown. We hypothesized that the oral epithelium itself may participate in sensing thermal, mechanical, and chemical conditions. The expression profiles of TRP channels exhibited regional differences among the buccal, palatal, and tongue epithelia. Changes in elevated intracellular Ca2+ concentration ([Ca2+]i) in oral epithelial cells were found after stimulation of the TRP channels with capsaicin, camphor, 4α-phorbol-12,13 didecanoate (4α-PDD), 2-aminoethoxydiphenyl borate (2-APB), and menthol. These increases in Ca2+ appeared dependent on the TRP channels, because [Ca2+]i suppression was observed after the addition of the TRPV channel antagonist ruthenium red. These results demonstrate that the oral epithelia express various TRP channels and may have functional roles in sensory activities, together with neurons.