Plastid protein synthesis is required for plant development in tobacco
Plastid protein synthesis is required for plant development in tobacco
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DOI：
10.1073/pnas.2533668100
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发表时间：
2003-12-23
影响因子：
11.1
通讯作者：
Bock, R
中科院分区：
文献类型：
--
作者：
Ahlert, D;Ruf, S;Bock, R
叶绿体在细胞代谢中起着重要的作用。大多数质体基因组编码的基因参与光合作用或叶绿体基因的表达。质体基因是否也能决定外质体功能一直存在争议。我们在这里证明了质体蛋白合成在烟叶发育中的重要作用。通过叶绿体转化，我们开发了一个实验系统，以细胞系特异性的方式产生基于重组的叶绿体翻译敲除。由此产生的植物是嵌合的，在翻译抑制剂的存在下，表现出严重的发育异常。在缺乏活性质体蛋白合成的情况下，由于细胞分裂明显停止，叶片发育被取消。这种效应似乎是细胞自主的，因为具有翻译质体的相邻细胞区在表型上是正常的，但不能弥补突变区中质体翻译的缺失。在花的形态上也可以看到发育异常，这表明缺陷不是由质体光合作用基因的抑制表达引起的。综上所述，我们的数据表明质体基因和基因表达在植物发育和细胞分裂中具有意想不到的重要作用。
Chloroplasts fulfill important functions in cellular metabolism. The majority of plastid genome-encoded genes is involved in either photosynthesis or chloroplast gene expression. Whether or not plastid genes also can determine extraplastidic functions has remained controversial. We demonstrate here an essential role of plastid protein synthesis in tobacco leaf development. By using chloroplast transformation, we have developed an experimental system that produces recombination-based knockouts of chloroplast translation in a cell-line-specific manner. The resulting plants are chimeric and, in the presence of translational inhibitors, exhibit severe developmental abnormalities. In the absence of active plastid protein synthesis, leaf blade development is abolished because of an apparent arrest of cell division. This effect appears to be cell-autonomous in that adjacent sectors of cells with translating plastids are phenotypically normal but cannot complement for the absence of plastid translation in mutant sectors. Developmental abnormalities also are seen in flower morphology, indicating that the defects are not caused by inhibited expression of plastid photosynthesis genes. Taken together, our data point to an unexpected essential role of plastid genes and gene expression in plant development and cell division.