Isolation of the human semaphorin III/F gene (SEMA3F) at chromosome 3p21, a region deleted in lung cancer
Isolation of the human semaphorin III/F gene (SEMA3F) at chromosome 3p21, a region deleted in lung cancer
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DOI：
10.1006/geno.1996.0074
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发表时间：
1996-02-15
期刊：
影响因子：
4.4
通讯作者：
Naylor, SL
中科院分区：
文献类型：
--
作者：
Xiang, RH;Hensel, CH;Naylor, SL
小细胞肺癌（SCLC）与3号染色体短臂的缺失相关，几乎所有病例中3 p21区域都从一个同源物中丢失。两个SCLC细胞系在3 p21中具有纯合缺失，并且这些缺失与在体内具有肿瘤抑制活性的染色体3的片段重叠。我们已经从这个区域分离出了一些与构成脑信号蛋白家族的基因同源的cDNA克隆。它们代表了一种新的人类脑信号蛋白，称为sema III/F（HGMW批准的符号SEMA 3F），在多种细胞系和组织中表达为3.8 kb的转录本;早在小鼠发育的胚胎第10天就被检测到。在乳腺、肾脏、胎脑和肺中表达较高，在心脏和肝脏中表达较低。尽管该基因在几个小细胞肺癌细胞系中表达减少，但未发现突变。该脑信号蛋白同源物具有脑信号蛋白III家族分泌成员的特征，与小鼠脑信号蛋白E具有52%的同一性，与鸡脑信号蛋白/脑信号蛋白D具有49%的同一性。(C)出版社：Academic Press，Inc.
Small cell lung cancer (SCLC) has been correlated with a deletion in the short arm of chromosome 3, with the region 3p21 being lost from one homolog in almost all cases. Two SCLC cell lines have homozygous deletions in 3p21, and these deletions overlap with a fragment of chromosome 3 that has tumor suppression activity in vivo. We have isolated some cDNA clones from this region that are homologous to the genes constituting the semaphorin family. They represent a novel human semaphorin, termed sema III/F (HGMW-approved symbol SEMA3F), which is expressed as a 3.8-kb transcript in a variety of cell lines and tissues; it is detected as early as Embryonic Day 10 in mouse development. There is high expression in mammary gland, kidney, fetal brain, and lung and lower expression in heart and liver. Although there is reduced expression of this gene in several SCLC lines, no mutations were found. This semaphorin homolog has characteristics of a secreted member of the semaphorin III family, with 52% identity with mouse semaphorin E and 49% identity with chicken collapsin/semaphorin D. (C) 1996 Academic Press, Inc.