The cytoreceptor assay for 1,25-dihydroxyvitamin D and its application to clinical studies.
The cytoreceptor assay for 1,25-dihydroxyvitamin D and its application to clinical studies.
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1,25-二羟基维生素 D 的细胞受体测定及其在临床研究中的应用。
DOI：
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发表时间：
1983
影响因子：
5.8
通讯作者：
L. Deftos
中科院分区：
文献类型：
--
作者：
S. Manolagas;F. Culler;J. Howard;A. Brickman;L. Deftos
我们已经开发了一种用于测量1，25-二羟基维生素D [1，25-（OH）2D]的方法，该方法基于1，25-（OH）2D在培养的靶细胞内选择性内化并随后与其特异性受体结合。该技术提供了直接测定血液提取物中1，25-（OH）2D的方法，而无需事先将该激素与维生素D的其他代谢物进行生化分离。通过提取的1，25-（OH）2D置换与细胞内受体结合的1，25-（OH）2-[3 H]D3，可测量其在血液样品中的浓度。该方法灵敏度为2 pg/管，可使用1- 2 ml样品进行。批内和批间变异系数分别为10.2%和15.3%。维生素D的其他主要代谢物在其循环水平下不会干扰1，25-（OH）2D测量。用该法测定的1，25-（OH）_2D含量与传统的高压液相色谱法测定的1，25-（OH）_2D含量具有良好的相关性（r = 0.93）。
We have developed a method for the measurement of 1,25-dihydroxyvitamin D [1,25-(OH)2D] which is based on the selective internalization and subsequent binding of 1,25-(OH)2D to its specific receptor inside cultured target cells. This technique provides the means of assaying 1,25-(OH)2D directly in blood extracts without prior biochemical isolation of this hormone from other metabolites of vitamin D. The displacement of 1,25-(OH)2-[3H]D3 bound to the intracellular receptor by the extracted 1,25-(OH)2D provides a measurement of its concentration in the blood samples. The method is sensitive to 2 pg/tube and can be performed with 1- to 2-ml samples. The intra- and interassay coefficients of variation are 10.2% and 15.3%, respectively. Other major metabolites of vitamin D, at their circulating levels, do not interfere with the 1,25-(OH)2D measurement. Moreover, 1,25-(OH)2D levels determined by this direct method correlate closely (r = 0.93) with the values determined by the traditional high pressure liquid ch...
影响因子：
8.9
作者：
Kumar,R;Riggs,BL
通讯作者：
Riggs,BL