The role of autophagy in THP‐1 macrophages resistance to HIV‐ vpr‐induced apoptosis
The role of autophagy in THP‐1 macrophages resistance to HIV‐ vpr‐induced apoptosis
复制标题
自噬在 THP-1 巨噬细胞抵抗 HIV-vpr 诱导的细胞凋亡中的作用
DOI：
10.1016/j.yexcr.2016.12.024
复制
发表时间：
2017
影响因子：
3.7
通讯作者：
B. He
中科院分区：
文献类型：
--
作者：
Hua‐ying Zhou;Yu;Yan He;Zi Chen;B. He
巨噬细胞对细胞死亡具有抵抗力，是艾滋病毒的储存库之一。HIV病毒蛋白VPR具有促进巨噬细胞感染和存活的潜能，在巨噬细胞病毒库的发育和/或维持中可能是一个非常重要的因素。然而，VPR对巨噬细胞抗凋亡的影响尚不清楚。自噬是细胞在应激状态下的一种生存机制。在这项研究中，我们研究了自噬是否参与了巨噬细胞对VPR诱导的凋亡的抵抗。利用THP1巨噬细胞，我们研究了巨噬细胞抗凋亡和自噬之间的相互关系。我们发现VPR能够触发THP-1巨噬细胞的自噬，电子显微镜(EM)和免疫印迹分析证实，3MA抑制自噬增加了VPR诱导的细胞凋亡。结果表明，自噬可能是维持巨噬细胞HIV储存库的原因之一。
Macrophages are resistant to cell death and are one of HIV reservoirs. HIV viral protein Vpr has the potential to promote infection of and survival of macrophages, which could be a highly significant factor in the development and/or maintenance of macrophage viral reservoirs. However, the impact of vpr on macrophages resistance to apoptosis is yet to be comprehended. Autophagy is a cell survival mechanism under stress state. In this study, we investigated whether autophagy is involved in macrophages resistant to vpr-induced apoptosis. Using the THP1 macrophages, we studied the interconnection between macrophages resistance to apoptosis and autophagy. We found that vpr is able to trigger autophagy in transfected THP-1 macrophages confirmed by electron microscopy (EM) and western blot analysis, and inhibition of autophagy with 3MA increased vpr-induced apoptosis. The results indicate that autophagy may be responsible for maintenance of macrophage HIV reservoirs.
影响因子：
2.2
作者：
Gillim-Ross, L;Cara, A;Klotman, ME
通讯作者：
Klotman, ME