Ras1CA overexpression in the posterior silk gland improves silk yield
Ras1CA overexpression in the posterior silk gland improves silk yield
复制标题
Ras1CA 在后丝腺中过度表达可提高丝产量
DOI：
10.1038/cr.2011.36
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发表时间：
2011-06-01
期刊：
影响因子：
44.1
通讯作者：
Li, Sheng
中科院分区：
文献类型：
--
作者：
Ma, Li;Xu, Hanfu;Li, Sheng
家蚕杂交育种技术的应用极大地促进了养蚕业的发展，但在过去几十年里，养蚕业已进入停滞期。我们首次报道了GAL 4/UAS转基因家蚕的产丝量提高。Ras 1 CA癌基因特异性地在后部丝腺中的过表达将丝素蛋白生产和丝产量提高60%，而食物消耗仅增加20%。Ras激活Ras 1 CA过表达在后部丝腺增强磷酸化水平的Ras下游效应蛋白，上调丝素蛋白mRNA水平，增加总DNA含量，并刺激内复制。此外，Ras 1的激活增加了细胞和细胞核的大小，丰富了与蛋白质合成相关的亚细胞器，并刺激了mRNA翻译的核糖体生物合成。我们的结论是，Ras 1激活增加细胞大小和蛋白质合成的后部丝腺，导致丝产量的提高。
Sericulture has been greatly advanced by applying hybrid breeding techniques to the domesticated silkworm, Bombyx mori, but has reached a plateau during the last decades. For the first time, we report improved silk yield in a GAL4/UAS transgenic silkworm. Overexpression of the Ras1 CA oncogene specifically in the posterior silk gland improved fibroin production and silk yield by 60%, while increasing food consumption by only 20%. Ras activation by Ras1 CA overexpression in the posterior silk gland enhanced phosphorylation levels of Ras downstream effector proteins, up-regulated fibroin mRNA levels, increased total DNA content, and stimulated endoreplication. Moreover, Ras1 activation increased cell and nuclei sizes, enriched subcellular organelles related to protein synthesis, and stimulated ribosome biogenesis for mRNA translation. We conclude that Ras1 activation increases cell size and protein synthesis in the posterior silk gland, leading to silk yield improvement.