Towards a systemic metabolic signature of the arbuscular mycorrhizal interaction
Towards a systemic metabolic signature of the arbuscular mycorrhizal interaction
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DOI：
10.1007/s00442-011-2037-6
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发表时间：
2011-12-01
期刊：
影响因子：
2.7
通讯作者：
Haertig, Claus
中科院分区：
文献类型：
--
作者：
Fester, Thomas;Fetzer, Ingo;Haertig, Claus
我们的实验解决了地上植物组织的系统代谢效应，作为植物对丛枝菌根（AM）相互作用的反应的一部分。由于这种相互作用的生理学，我们预计在植物矿物质营养，碳分配和胁迫相关代谢领域的影响，以及各自的代谢变化对环境条件和植物发育计划的显着依赖。为了评估这些问题，我们分析了在温室条件下生长的菌根和非菌根日本莲在生长季节的三个不同时间点上的三个不同地上器官（花、汇叶和源叶）的代谢物谱。我们的数据统计分析揭示了一些显著的变化在个别实验中，这些实验之间几乎没有重叠，表明外界条件对植物对AM定植的反应的预期影响。然而，偏最小二乘判别分析（PLS-DA）揭示了数据集之间相当大的相似性，并且对分离菌根和非菌根植物的成分进行加载分析，允许定义负责这种分离的核心代谢物集。在有和没有菌根诱导生长效应的实验中观察了该核心组。它证实了个体实验的显著变化所表明的趋势，并表明AM定殖对中枢分解代谢和氨基酸代谢有负面的全身影响。此外，在花和源叶中记录了植物组织所经历的胁迫增加的代谢信号。
Our experiments addressed systemic metabolic effects in above-ground plant tissue as part of the plant's response to the arbuscular mycorrhizal (AM) interaction. Due to the physiology of this interaction, we expected effects in the areas of plant mineral nutrition, carbon allocation and stress-related metabolism, but also a notable dependence of respective metabolic changes on environmental conditions and on plant developmental programs. To assess these issues, we analyzed metabolite profiles from mycorrhizal and non-mycorrhizal Lotus japonicus grown under greenhouse conditions at three different time points in the growing season in three different above-ground organs (flowers, sink leaves and source leaves). Statistical analysis of our data revealed a number of significant changes in individual experiments with little overlap between these experiments, indicating the expected impact of external conditions on the plant's response to AM colonization. Partial least square-discriminant analysis (PLS-DA) nevertheless revealed considerable similarities between the datasets, and loading analysis of the component separating mycorrhizal and non-mycorrhizal plants allowed the defining of a core set of metabolites responsible for this separation. This core set was observed in experiments with and without mycorrhiza-induced growth effects. It corroborated trends already indicated by the significant changes from individual experiments and suggested a negative systemic impact of AM colonization on central catabolic metabolism as well as on amino acid metabolism. In addition, metabolic signals for an increase in stress experienced by plant tissue were recorded in flowers and source leaves.