TRICHINELLA-SPIRALIS - SECRETED ANTIGEN OF THE INFECTIVE L1 LARVA LOCALIZES TO THE CYTOPLASM AND NUCLEOPLASM OF INFECTED HOST-CELLS
TRICHINELLA-SPIRALIS - SECRETED ANTIGEN OF THE INFECTIVE L1 LARVA LOCALIZES TO THE CYTOPLASM AND NUCLEOPLASM OF INFECTED HOST-CELLS
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DOI：
10.1016/0014-4894(90)90005-w
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发表时间：
1990-07-01
影响因子：
2.1
通讯作者：
SILBERSTEIN, D
中科院分区：
文献类型：
--
作者：
DESPOMMIER, DD;GOLD, AM;SILBERSTEIN, D
针对表观分子量为43 K的纯化抗原引发抗体。该抗体制剂还检测到由一组45- 50 K的密切相关组分组成的第二抗原。这些抗原对旋毛虫感染性第一阶段幼虫具有阶段特异性，并且是源自丝体的分泌抗原库之一。对43 K抗原的抗体提出了反应的stichosome和角质层的成熟幼虫和细胞质和核仁，但不是核仁，所有的细胞核的感染宿主细胞（护士细胞）在受感染的组织切片。对同步感染肌肉组织切片的研究表明，在感染第7天，抗原仅存在于蠕虫内。感染后第9天，幼虫的化学小体和表皮表面以及护士细胞各核的细胞质和核质与抗体反应。直到感染后第18天，护士细胞的细胞质和核反应强度增加。这些结果表明，stichocyte-specific抗原在肌肉中的感染的早期阶段期间合成，并且作为护士寄生虫复合体的发展，一些抗原分泌到护士细胞的环境中。讨论了与护士细胞形成和维持有关的感染宿主细胞的细胞质和核质中抗原的存在。
Antibodies were elicited against a purified antigen with an apparent molecular weight of 43K. This antibody preparation also detected a second antigen consisting of a group of closely related components of 45-50K. These antigens are stage specific for the infective first stage larva of Trichinella spiralis and are among the repertoire of secreted antigens originating from the stichosome. Antibody raised against the 43K antigen reacted with the stichosome and cuticle of the mature larva and the cytoplasm and nucleoplasms, but not nucleolus, of all nuclei of infected host cells (Nurse cells) in sections of infected tissues. Studies on sections of syncrhonously infected muscle tissue revealed that antigen was present only within the worm on Day 7 of the infection. On Day 9 after infection, the stoichosome and cuticular surface of the larva and the cytoplasm and nuceloplasm of each nucleus of the Nurse cell reacted with antibody. Nurse cell cytoplasmic and nuclear reactivity increased in intensity until Day 18 after infection. These results suggest that stichocyte-specific antigens are synthesized during the early phase of infection in the muscle, and that as the Nurse-parasite complex develops, some of the antigen is secreted into the milieu of the Nurse cell. The presence of antigen in the cytoplasm and nucleoplasm of the infected host cell is discussed in relation to Nurse cell formation and maintenance.