Effects of concomitant diabetes mellitus and hyperthyroidism on testicular and epididymal histoarchitecture and steroidogenesis in male animals
Effects of concomitant diabetes mellitus and hyperthyroidism on testicular and epididymal histoarchitecture and steroidogenesis in male animals
复制标题
糖尿病和甲状腺功能亢进对雄性动物睾丸和附睾组织结构以及类固醇生成的影响
DOI：
10.1631/jzus.b1600136
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发表时间：
2016-11-01
影响因子：
5.1
通讯作者：
Shi, Fang-xiong
中科院分区：
文献类型：
--
作者：
Korejo, Nazar Ali;Wei, Quan-wei;Shi, Fang-xiong
This study evaluated the effects of comorbid disorders of diabetes and hyperthyroidism in the adult male mice. In total, 32 ICR strain mice were equally distributed into four groups: control (C), diabetic (D), diabetic-plushyperthyroid (DH), and hyperthyroid (H). Mice allocated for diabetes received a single intraperitoneal injection of streptozotocin (STZ) at 200 mg/kg body weight. At the onset of diabetes, one group of mice was concomitantly injected levothyroxine (LT4; 0.3 mg/kg body weight) and the other set of animals received the same treatment independently on a daily basis. The body weight, as well as the testicular and epididymal weights, was reduced markedly in D and DH mice. Higher trends of blood glucose levels were seen in the DH group, in comparison to euthyroid diabetic mice. Thyroid hormones could exert a transient effect on blood glucose homeostasis by altering the serum blood glucose level in diabetic patients. Histomorphometric analysis showed increased luminal sizes of seminiferous tubules, along with decreased epithelial height and atrophic changes in germinal stem cells in the testis of DH and H mice. Caput epididymis of DH mice showed extensive compaction of principal cells, loss of stereocilia, lipid vacuolization, and inflammatory infiltrations; however, damaged tubular integrity, packed clear cells, exfoliated cells, and round spermatids were profoundly noticed in the cauda epididymis. Hyperthyroidism elevated the serum testosterone levels in H and DH mice and produced critical damages to the histoarchitecture of the epididymis. Collectively, this experiment endeavored to mimic the polyglandular autoimmune syndrome, which will be helpful to better understand the reasons for male infertility in diabetic-cum-hyperthyroid patients.概要目 的评估糖尿病和甲状腺功能亢进对雄性动物睾丸和 附睾组织形态学及类固醇激素合成的影响, 并初 步探讨其作用机制。创新点以小鼠为模型, 首次研究并发糖尿病和甲状腺功 能亢进对雄性哺乳动物睾丸、附睾发育和类固醇 激素合成的影响。方 法32 只ICR 品系小鼠分为四组: 对照组(C)、糖 尿病组(D)、糖尿病+甲亢组(DH) 和甲亢组 (H)。D 组小鼠以200 mg/kg 剂量单次腹膜内注 射链脲佐菌素(STZ), 诱导糖尿病成功。另对 其中一半以0.3 mg/kg 剂量每天注射甲状腺素, 组成DH 组。小鼠试验结束后, 采集睾丸、附睾 和血液, 并离心分离获得血清。睾丸和附睾用4% (0.04 g/ml) 多聚甲醛固定, 并用苏木精-伊红染 色法(H & E) 观察睾丸和附睾组织形态学变化, 用放射免疫测定(RIA) 试剂盒检测血清中睾酮、 促甲状腺激素(TSH)、胰岛素、甲状腺素(T4) 和三碘甲状腺原氨酸(T3) 的含量并进行分析。结 论D 和DH 组小鼠的体重、睾丸和附睾的重量显著 降低。相比于正常甲亢或糖尿病小鼠, DH 组中 血糖水平显著升高。甲状腺激素可能是通过改变 糖尿病患者的血清血糖水平对血糖稳态产生瞬 时影响。 组织形态学分析结果显示, 在DH 和H 组小鼠睾丸中, 输精管管腔增大, 上皮厚度减少, 睾丸生殖干细胞发生萎缩性变化。DH 组小鼠的 附睾头呈现主细胞压实、纤毛、脂质空泡化和炎 症浸润现象。在附睾尾部观察到了小管完整性受 损、透明细胞聚积和细胞脱落, 并发现圆形精子。 对于DH和H 组, 甲亢提高了小鼠血清睾酮水平, 并损害了附睾的组织形态。总之, 本试验模拟了 多腺体自身免疫综合征对雄性繁殖的影响, 这将 有助于更好地了解男性并发糖尿病和甲亢患者 不育的原因。