Characterization of Internal Transcribed Spacer (ITS1)-ITS2 Region of Ribosomal RNA Gene From 25 Species of Culicoides Biting Midges (Diptera: Ceratopogonidae) in Japan
Characterization of Internal Transcribed Spacer (ITS1)-ITS2 Region of Ribosomal RNA Gene From 25 Species of Culicoides Biting Midges (Diptera: Ceratopogonidae) in Japan
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DOI：
10.1603/033.046.0517
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发表时间：
2009-09-01
影响因子：
2.1
通讯作者：
Noda, H.
中科院分区：
文献类型：
--
作者：
Matsumoto, Y.;Yanase, T.;Noda, H.
测定了日本11个地点25种库蠓（Culicoides）1.03个个体的rDNA内转录间隔区（ITS）1-5. 8 S-ITS 2a-2S-ITS 2区域的核苷酸序列。核糖体RNA基因5.8S和2S rDNA在种间高度保守，变异较少。ITS 2a区域在物种之间表现出长度变化。ITS 1和ITS 2在物种间均表现出高度变异的序列。ITS I序列间的indel区在库蠓属的某些种中存在，将库蠓属的种分为ITS 1区长和ITS 1区短的两种类型。然而，Culicoides cylindratus Kitaoka在每个个体中都具有两种类型的ITS 1;这些结果似乎表明，ITS 1长型是原型，而短型是通过许多库蠓物种的缺失产生的。其中一个种属于Avaritia亚属，在ITS 1中具有Avaritia特有序列，并在遗传学上形成了一个单系群。一个物种的地理基因型不清楚。观察到种特异性序列特征，使库蠓种的分子鉴定。
We determined nucleotide sequences of the nuclear rDNA internal transcribed spacer (ITS) 1-5.8S-ITS2a-2S-ITS2 region in 1.03 individuals of 25 Culicoides species (Diptera: Ceratopogonidae) from 11 locations in Japan. Ribosomal RNA genes, 5.8S and 2S rDNA, were highly conserved among the species with few variations. The ITS2a region showed length variation among species. Both ITS1 and ITS2 showed highly varied sequences among species. The noticeable indel regions among ITS I sequences are present in some Culicoides species, separating species into two types having long or short ITS1 region. However, Culicoides cylindratus Kitaoka possesses both types of ITS1 in each individual; these results seem to indicate that the ITS1-long type was the prototype and the short type was produced through deletion in many Culicoides species. One species, belonging to, p subgenus Avaritia, possessed an Avaritia-specific sequence in ITS1 and phylogenetically formed a monophyletic group. Geographical genotypes in a species were not clear. Species-specific sequence features were observed, enabling molecular identification of Culicoides species.