Rapid identification of erythrocyte phospholipids in Sprague-Dawley rats by ultra high performance liquid chromatography with electrospray ionization quadrupole time-of-flight tandem mass spectrometry.
Rapid identification of erythrocyte phospholipids in Sprague-Dawley rats by ultra high performance liquid chromatography with electrospray ionization quadrupole time-of-flight tandem mass spectrometry.
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DOI：
10.1002/jssc.201401120
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发表时间：
2015-03
影响因子：
3.1
通讯作者：
Juanjuan Pi;Xia Wu;Shitian Yang;Pingyan Zeng;Y. Feng
中科院分区：
文献类型：
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作者：
Juanjuan Pi;Xia Wu;Shitian Yang;Pingyan Zeng;Y. Feng
采用超高效液相色谱-电喷雾四极杆飞行时间串联质谱法，以MassLynx（TM）MassFragment为分析工具，建立了一种快速、灵敏、可靠的测定SD大鼠红细胞5种磷脂的方法。超高效液相色谱与四极杆飞行时间串联质谱联用技术改进了传统的高效液相色谱技术，通过使用填充有亚2 μ m颗粒（1.7 μ m）的色谱柱，可实现更快的分离，从而提供高灵敏度和更快的分析速度。通过该方法，基于质谱和串联质谱，以及通过在5 ppm的质量误差内匹配内部配方数据库，初步表征了83种磷脂，包括40种磷脂酰胆碱、24种磷脂酰乙醇胺、3种磷脂酰肌醇、6种磷脂酰丝氨酸和10种鞘磷脂。本研究结果表明，所建立的方法可用于Sprague-Dawley大鼠复杂红细胞磷脂的定性分析，为药理学和磷脂组学研究提供了有用的数据基础，为寻找潜在的疾病预测生物标志物提供了依据。
A rapid, sensitive, and reliable approach for analyzing five kinds of erythrocyte phospholipids in Sprague-Dawley rats was provided by ultra high performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry with MassLynx(TM) MassFragment. Improving conventional high performance liquid chromatography techniques, ultra high performance liquid chromatography integrated with quadrupole time-of-flight tandem mass spectrometry offers high sensitivity and increased analytical speed by using columns packed with sub-2 μm particles (1.7 μm), which allows a faster separation to be achieved. Through this method, 83 phospholipids were tentatively characterized based on their mass spectra and tandem mass spectra, as well as by matching the in-house formula database within a mass error of 5 ppm, including 40 phosphatidylcholines, 24 phosphatidyl ethanolamines, three phosphatidylinositols, six phosphatidylserines, and ten sphingomyelins. Our present results proved that the established method could be used to qualitatively analyze complex erythrocyte phospholipids in Sprague-Dawley rats and provide a useful data base for pharmacology and phospholipidomics to seek potential biomarkers of disease prediction.