Bio-microfluidic platform for gold nanoprobe based DNA detection-application to Mycobacterium tuberculosis
Bio-microfluidic platform for gold nanoprobe based DNA detection-application to Mycobacterium tuberculosis
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DOI：
10.1016/j.bios.2013.03.079
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发表时间：
2013-10-15
影响因子：
12.6
通讯作者：
Martins, Rodrigo
中科院分区：
文献类型：
--
作者：
Bernacka-Wojcik, Iwona;Lopes, Paulo;Martins, Rodrigo
我们已经设计并制造了一个用于DNA传感的微流控平台，该平台利用了基于金纳米颗粒的光学比色检测方法。该平台采用高纵横比SU-8模具在PDMS上复制成型技术制造。为了找出最有效的结构，对不同几何形状的生物芯片进行了测试和评估，并提出了设计、微加工和检测性能的合理性。最佳生物芯片配置已成功应用于仅使用3 μ l DNA溶液（即90 ng靶DNA）检测结核分枝杆菌，因此与现有技术相比，试剂体积减少了20倍。(C) 2013 Elsevier B.V.版权所有
We have projected and fabricated a microfluidic platform for DNA sensing that makes use of an optical colorimetric detection method based on gold nanoparticles. The platform was fabricated using replica moulding technology in PDMS patterned by high-aspect-ratio SU-8 moulds. Biochips of various geometries were tested and evaluated in order to find out the most efficient architecture, and the rational for design, microfabrication and detection performance is presented. The best biochip configuration has been successfully applied to the DNA detection of Mycobacterium tuberculosis using only 3 mu l on DNA solution (i.e. 90 ng of target DNA), therefore a 20-fold reduction of reagents volume is obtained when compared with the actual state of the art. (C) 2013 Elsevier B.V. All rights reserved.