A morphological and electron-microprobe study of the inorganic composition of the mineralized secretory products of the calciferous gland and chloragogenous tissue of the earthworm, Lumbricus terrestris L.
A morphological and electron-microprobe study of the inorganic composition of the mineralized secretory products of the calciferous gland and chloragogenous tissue of the earthworm, Lumbricus terrestris L.
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对蚯蚓（Lumbricus terrestris L.）钙质腺和产绿组织矿化分泌产物无机成分的形态学和电子显微镜研究。
DOI：
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发表时间：
1981
影响因子：
3.6
通讯作者：
Dr. A. J. Morgan
中科院分区：
文献类型：
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作者：
Dr. A. J. Morgan
蚯蚓含钙腺的两对叶是特化的食管憩室，分泌直径为0.5-7.0 μm的小球。相关的透射和扫描电子显微镜表明，（主要是CaCO 3）在细胞外形成于由分泌细胞突起界定的独特的海湾中，并且从腺腔向前移动到非分泌囊的腔，其中大多数聚结并经历相变为结核0.5-直径为1.0 mm，由大量立方晶体组成，小平面最大可达40 μm。将0.1 ml 5%SrCl 2注入后体腔，用扫描电镜对10 μm厚的冷冻切片进行X射线微区分析，观察Sr在含钙腺体矿化分泌产物中的分布。在注射后2 h和24 h，在氯质体中未检测到锶。锶被向前运输，并特异性地掺入腺球（2小时内可检测到）。Sr定位技术提供了足够的结构和分析分辨率，以确认腺体/囊系统中细胞外变化的顺序。此外，我们能够区分人口的增长spherites从绝大多数成熟spherites;大小单独是一个非常贫穷的指标spherites growth.The主要元素成分的chloragosomes是P，Ca和Zn（Ca：P范围从0.4到1.0; Zn：P从约0.05到0.45）。个别球粒的分析表明，钙可能是绑定到P或含P的基质成分，而锌可能是一个或多个不同的，但未知的成分。
SummaryThe two pairs of lobes of the calciferous gland of the earthworm Lumbricus terrestris are specialized oesophageal diverticulae that secrete spherites ranging from 0.5–7.0 μm in diameter. Correlative transmission and scanning electron microscopy indicated that the spherites (which are predominantly CaCO3) are formed extracellularly in distinctive bays bounded by secretory-cell processes, and are mobilized anteriorly from the gland lumina to the lumen of a non-secretory pouch, where the majority coalesce and undergo phase transformation to concretions 0.5–1.0 mm in diameter consisting of a mass of cuboidal crystals with facets up to 40 μm. The distribution of Sr (0.1 ml 5 % SrCl2 injected into the posterior coelomic cavity) was monitored in the mineralized secretory products of the calciferous glands by X-ray microanalysis of 10 μm — thick air-dried cryostat sections in a SEM. Strontium was not detected in chloragosomes at 2 h and 24 h post-injection. Strontium was transported anteriorly and specifically incorporated into gland spherites (detectable within 2h). This technique of Sr localization afforded sufficient structural and analytical resolutions to provide a confirmation of the sequence of extracellular changes in the gland/pouch system. In addition we were able to distinguish a population of growing spherites from the vast majority of mature spherites; size alone was a singularly poor indication of spherite growth.The major element constituents of the chloragosomes were P, Ca and Zn (Ca: P ranging from 0.4 to 1.0; Zn: P from about 0.05 to 0.45). Analysis of individual spherites showed that Ca was probably bound to P or P-containing matrix components, whilst Zn was probably linked to one or more different but unknown constituents.