Efficient secretion of the model antigen M6-gp41E in Lactobacillus plantarum NCIMB 8826
Efficient secretion of the model antigen M6-gp41E in Lactobacillus plantarum NCIMB 8826
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DOI：
10.1099/00221287-143-8-2733
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发表时间：
1997-08-01
期刊：
影响因子：
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通讯作者：
Mercenier, A
中科院分区：
文献类型：
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作者：
Hols, P;Slos, P;Mercenier, A
四株乳杆菌(Lb.Plantarum NCIMB 8826，lb.Paracasei LbTGS1.4，磅Casei ATCC 393和Lb.对它们产生和分泌异源蛋白的能力进行了测试。这些菌株首先在各种乳酸菌的一组表达或表达/分泌信号的控制下，用a-淀粉酶报告进行筛选。在测试的大多数结构中，Lb的胞外生产水平最高。Plantarum NCIMB 8826，最低为Lb.Paracasei LbTGS1.4.然后用化脓性链球菌M6蛋白的N端部分与人类免疫缺陷病毒gp41蛋白的线性表位ELDKWa融合的模型抗原对这两株菌株进行检测。在lb中，这种异源蛋白的分泌效率很低。Paracasei LbTGS1.4，积累了大量的未加工前体细胞内库。而Lb.该菌能分泌高达10 mg L(-1)的抗原。
Four Lactobacillus strains (Lb. plantarum NCIMB 8826, Lb. paracasei LbTGS1.4, Lb. casei ATCC 393 and Lb. fermentum KLD) were tested for their ability to produce and secrete heterologous proteins. These strains were first screened with an a-amylase reporter under the control of a set of expression or expression/secretion signals from various lactic acid bacteria. With most of the constructions tested, the lever of extracellular production was highest in Lb. plantarum NCIMB 8826, and lowest in Lb. paracasei LbTGS1.4. These two strains were next assayed using a model antigen consisting of the N-terminal part of the M6 protein from Streptococcus pyogenes fused to the linear epitope ELDKWAS from human immunodeficiency virus gp41 protein. Secretion of this heterologous protein was inefficient in Lb. paracasei LbTGS1.4 which accumulated a large intracellular pool of the unprocessed precursor. whereas Lb. plantarum NCIMB 8826 was able to secrete the antigen to a revel as high as 10 mg l(-1).