The study on role of peripheral blood mononuclear cell in HBV intrauterine infection
The study on role of peripheral blood mononuclear cell in HBV intrauterine infection
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外周血单个核细胞在HBV宫内感染中作用的研究
DOI：
10.1007/s00404-010-1366-8
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发表时间：
2011-02-01
影响因子：
2.6
通讯作者：
Shi, L.
中科院分区：
文献类型：
--
作者：
Bai, G. Q.;Li, S. H.;Shi, L.
为探讨母亲外周血单个核细胞(PBMC)感染乙肝病毒(HBVDNA)在宫内传播中的作用，我们选择了60例血清HBVDNA阴性、PBMC HBVDNA阳性的住院孕妇。这些妇女和她们的新生儿作为试验组。选择乙肝病毒血清标志物阴性孕妇20例。这些妇女和她们的新生儿作为对照组。采用免疫组织化学方法检测新生儿PBMC HBVDNA阳性和(或)新生儿血清HBVDNA阳性孕妇胎盘各层细胞和胎盘CD68细胞中HBSAg和HBcAg的表达。仅8例PBMC-HBVDNA阳性的新生儿胎盘绒毛间质和毛细血管CD68细胞表达HBSAg和HBcAg。新生儿血清HBVDNA阳性的4例中，2例胎盘各层细胞均有HBVDNA感染。4例新生儿血清和PBMC中HBVDNA均阳性的4例中，2例滋养层细胞、绒毛间质CD68细胞和毛细血管内均可检测到HBSAg和HBcAg的表达。对照组新生儿和胎盘均未检测到阳性信号，孕妇感染HBVPBMC可能导致宫内感染。
To explore the role of mother's peripheral blood mononuclear cell (PBMC) infected with hepatitis B virus (HBV) in intrauterine transmission.We have selected 60 cases of pregnant women with negative serum HBV DNA and positive PBMC HBV DNA from hospitalized patients. These women and their neonates acted as the experimental group. Twenty cases of pregnant women with HBV serum marker negative were selected. These women and their neonates served as the control group. Immunohistochemistry was employed to detect the expressions of HBsAg and HBcAg in cells of every placental layer and CD68 cells of placenta of the pregnant women whose neonates' PBMC HBV DNA was positive and/or whose neonates' serum HBV DNA positive.In the experimental group, neonatal serum HBV DNA of only four cases were positive, only eight cases' neonatal PBMC HBV DNA were positive and four cases had HBV DNA positive in both neonatal serum and PBMC. The expressions of HBsAg and HBcAg were detected in CD68 cells of villous stroma and blood capillary in only eight cases of neonatal placenta with positive PBMC HBV DNA. HBV infection was found in cells of every layer in placenta in two of four cases with neonatal serum HBV DNA positive. The expressions of HBsAg and HBcAg were detected in trophoblastic cells, CD68 cells of villous stroma and blood capillary in two of four cases with HBV DNA positive in both neonatal serum and PBMC. In control group, no positive signals were detected in neonates and placenta.HBV-infected PBMC in pregnant women may lead to intrauterine infection.