Dynamics of memory T cell proliferation under conditions of heterologous immunity and bystander stimulation
Dynamics of memory T cell proliferation under conditions of heterologous immunity and bystander stimulation
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DOI：
10.4049/jimmunol.169.1.90
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发表时间：
2002-07-01
影响因子：
4.4
通讯作者：
Selin, LK
中科院分区：
文献类型：
--
作者：
Kim, SK;Brehm, MA;Selin, LK
通过检测过继转移的CSFE标记的淋巴细胞性脉络丛脑膜炎病毒（LCMV）-免疫供体T细胞在Thy-1同源宿主中接种病毒或细胞因子诱导剂poly（I：Q），发现真正的记忆T细胞对不同刺激的反应显著不同。Poly（I：Q）（细胞因子）刺激引起对五个LCMV表位中的每一个特异性的记忆CD 8 T细胞的有限同步分裂，没有增加，有时在数量上损失，并且它们的表位层级没有变化。Homembryonic LCMV感染引起对每个表位特异性的T细胞超过七次分裂，数量急剧增加，层次结构发生微小变化。与异源病毒Pichinde和牛痘（VV）的感染引起超过7次分裂和增加的T细胞的数量，具体到一些puppatients交叉反应，但不是其他表位，并导致在LCMV特异性T细胞的层次结构的实质性变化。因此，可以存在记忆T细胞分裂而不增殖（即，细胞数量增加），以及在存在来自同源或异源病毒的Ag的情况下伴随增殖的分裂。病毒之间的异源保护性免疫不一定是相互的，因为LCMV保护VV，但VV不保护LCMV。VV引起LCMV诱导的CD 8和CD 4 T细胞的增殖，而LCMV不引起VV诱导的T细胞的增殖。因此，取决于病原体和感染的顺序，异源剂可以选择性地以与异源免疫一致的模式刺激记忆库。
By examining adoptively transferred CSFE-labeled lymphocytic choriomeningitis virus (LCMV)-immune donor T cells in Thy-1 congenic hosts inoculated with viruses or with the cytokine inducer poly(I:Q, strikingly different responses of bona fide memory T cells were found in response to different stimuli. Poly(I:Q (cytokine) stimulation caused a limited synchronized division of memory CD8 T cells specific to each of five LCMV epitopes, with no increase and sometimes a loss in number, and no change in their epitope hierarchy. Homologous LCMV infection caused more than seven divisions of T cells specific for each epitope, with dramatic increases in number and minor changes in hierarchy. Infections with the heterologous viruses Pichinde and vaccinia (VV) caused more than seven divisions and increases in number of T cells specific to some putatively cross-reactive but not other epitopes and resulted in substantial changes in the hierarchy of the LCMV-specific T cells. Hence, there can be memory T cell division without proliferation (i.e., increase in cell number) in the absence of Ag and division with proliferation in the presence of Ag from homologous or heterologous viruses. Heterologous protective immunity between viruses is not necessarily reciprocal, given that LCMV protects against VV but VV does not protect against LCMV. VV elicited proliferation of LCMV-induced CD8 and CD4 T cells, whereas LCMV did not elicit proliferation of VV-induced T cells. Thus, depending on the pathogen and the sequence of infection, a heterologous agent may selectively stimulate the memory pool in patterns consistent with heterologous immunity.