Quantitative trait loci mapping for yield and its components by using two immortalized populations of a heterotic hybrid in Gossypium hirsutum L.
Quantitative trait loci mapping for yield and its components by using two immortalized populations of a heterotic hybrid in Gossypium hirsutum L.
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DOI：
10.1007/s11032-011-9547-0
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发表时间：
2012-02-01
影响因子：
3.1
通讯作者：
Zhang, Tianzhen
中科院分区：
文献类型：
--
作者：
Liu, Renzhong;Wang, Baohua;Zhang, Tianzhen
数量性状基因座 (QTL) 作图为揭示陆地棉产量和产量组成部分以及杂种优势的遗传基础提供了强大的工具。本研究基于增加的表达序列标签-简单序列重复标记，重建了香杂棉2号（Gossypium hirsutum L.）重组自交系（RIL）的分子连锁图谱。 RIL 和通过 RIL 之间的交互产生的永生化 F2 (IF2) 都在多种环境下生长。测量了籽棉产量、皮棉产量、棉铃/株、棉铃重量、皮棉率、种子指数、皮棉指数和果枝数等产量和产量组成部分，并通过复合区间作图（CIM）方法在不同环境中重复鉴定其QTL。在总共 111 个非冗余 QTL 中，两个群体中均检测到 23 个。同时，多标记联合分析表明，其中16个QTL具有显着的环境相互作用。相关性状的QTL往往是并置的，籽棉产量和皮棉产量的大部分QTL与至少一个产量组成部分的QTL相关，这与相关分析中观察到的结果一致。对于许多具有显着加性效应的 QTL，来自产量性能较低的劣质亲本 CRI12 的阳性等位基因与性状改良相关。 IF2的性状表现和大量具有正显性效应的QTL表明显性在湘杂棉2号杂种优势的遗传基础中发挥着重要作用，非加性遗传也是群体衣分的重要遗传模式。这些QTL可为陆地棉分子标记辅助育种提供依据。
Quantitative trait loci (QTL) mapping provides a powerful tool for unraveling the genetic basis of yield and yield components as well as heterosis in upland cotton. In this research, a molecular linkage map of Xiangzamian 2 (Gossypium hirsutum L.)-derived recombinant inbred lines (RILs) was reconstructed based on increased expressed sequence tag-simple sequence repeat markers. Both the RILs and immortalized F2s (IF2) developed through intermating between RILs were grown under multiple environments. Yield and yield components including seed-cotton yield, lint yield, bolls/plant, boll weight, lint percentage, seed index, lint index and fruit branch number were measured and their QTL were repeatedly identified across environments by the composite interval mapping (CIM) method. From a total of 111 non-redundant QTL, 23 were detected in both two populations. In the meantime, multi-marker joint analyses showed that 16 of these QTL had significant environmental interaction. QTL for correlated traits tended to be collocated and most of the QTL for seed-cotton yield and lint yield were associated with QTL for at least one yield component, consistent with the results observed in correlation analyses. For many QTL with significant additive effects, positive alleles from CRI12, the inferior parent with lower yield performance, were associated with trait improvement. Trait performance of IF2s and the large number of QTL with positive dominant effects implied that dominance plays an important role in the genetic basis of heterosis in Xiangzamian 2 and that non-additive inheritance is also an important genetic mode for lint percentage in the population. These QTL can provide the bases for marker-assisted breeding programs of upland cotton.