Differential calcium dependence in the activation of c-Jun kinase and mitogen-activated protein kinase by muscarinic acetylcholine receptors in rat 1a cells.
Differential calcium dependence in the activation of c-Jun kinase and mitogen-activated protein kinase by muscarinic acetylcholine receptors in rat 1a cells.
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大鼠 1a 细胞中毒蕈碱乙酰胆碱受体激活 c-Jun 激酶和丝裂原激活蛋白激酶的差异钙依赖性。
DOI：
10.1042/bj3090381
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Johnson,GL
中科院分区：
文献类型：
--
作者：
Mitchell,FM;Russell,M;Johnson,GL
在大鼠1a成纤维细胞中稳定表达的毒蕈碱乙酰胆碱m1受体（m1 R）的卡巴胆碱刺激导致c-Jun激酶（JNK）的钙依赖性激活。毒蕈碱乙酰胆碱m2受体（m2 R），稳定表达在大鼠1a成纤维细胞的刺激，导致G1介导的激活JNK是弱相对于观察到的m1 R。螯合钙抑制m2 R介导的JNK活化，但不抑制m2 R对丝裂原活化蛋白激酶（MAPK）活性的强烈刺激。这些研究结果表明，在大鼠1a细胞的JNK和MAPK的活性的差异调节的第二信使，钙的作用。
Carbachol stimulation of the muscarinic acetylcholine m1 receptor (m1R), stably expressed in Rat 1a fibroblasts, resulted in a calcium-dependent activation of c-Jun kinase (JNK). Stimulation of the muscarinic acetylcholine m2 receptor (m2R), stably expressed in Rat 1a fibroblasts, resulted in a G1-mediated activation of JNK that was weak relative to that observed with the m1R. Chelation of calcium inhibited the m2R-mediated activation of JNK but not the robust m2R stimulation of mitogen-activated protein kinase (MAPK) activity. These findings demonstrate a role for the second messenger, calcium, in the differential regulation of the activity of JNK and MAPK in Rat 1a cells.