Effect of fatty acids on boar sperm motility viability and acrosome reaction
Effect of fatty acids on boar sperm motility viability and acrosome reaction
复制标题
脂肪酸对公猪精子活力和顶体反应的影响
DOI：
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发表时间：
2007
影响因子：
3.4
通讯作者：
H. Tsujii
中科院分区：
文献类型：
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作者：
Sharoare Hossain;K. Tareq;Ko;H. Tsujii
AimThe目前的研究进行，以确定哪些脂肪酸提高运动活力和增加顶体反应（AR）在猪spermatozoes.MethodsBoar精子洗涤，swum-up和孵育在37°C的TALP培养基中4小时，补充肉豆蔻酸，棕榈酸，硬脂酸，二十四烷酸，油酸，亚油酸，花生四烯酸，二十二碳六烯酸和棕榈油酸。结果油酸和亚油酸能显著提高猪精子的活力和存活率（P <0. 05）。油酸和花生四烯酸在几乎所有孵育期均能显著提高AR（P < 0.05）。结果表明，油酸+亚油酸显著提高了猪精子的活力（P < 0.05），而花生四烯酸+油酸显著提高了猪精子的AR（P <0.05）。结论不饱和脂肪酸，尤其是花生四烯酸能提高猪精子的活力和AR。花生四烯酸和油酸的组合对于诱导公猪精子AR是重要的。
AimThe present study was undertaken to determine which fatty acids improve motility viability and increase acrosome reaction (AR) in boar spermatozoa.MethodsBoar spermatozoa were washed, swum-up and incubated at 37°C for 4 h in TALP medium supplemented with myristic, palmitic, stearic, lignoceric, oleic, linoleic, arachidonic, docosahexaenoic and palmitoleic acid. Sperm motility, viability and AR were evaluated during 4 h of incubation.ResultsResults show that oleic and linoleic acid significantly improved (P < 0.05) the motility and viability of boar spermatozoa. The AR was significantly improved (P < 0.05) by oleic and arachidonic acid in almost all incubation periods. When combinations of oleic, linoleic and arachidonic acid were studied for motility, viability and AR, it was found that oleic plus linoleic acid significantly increased (P < 0.05) motility, whereas arachidonic plus oleic acid significantly increased (P < 0.05) AR.ConclusionUnsaturated fatty acids, especially arachidonic acid, can improve boar sperm motility and AR. A combination of arachidonic and oleic acid is important for inducing boar sperm AR.