Analysis of OPLA scaffolds for bone engineering constructs using human jaw periosteal cells
Analysis of OPLA scaffolds for bone engineering constructs using human jaw periosteal cells
复制标题
DOI：
10.1007/s10856-007-3351-8
复制
发表时间：
2008-03-01
影响因子：
3.7
通讯作者：
Reinert, Siegmar
中科院分区：
文献类型：
--
作者：
Alexander, Dorothea;Hoffmann, Juergen;Reinert, Siegmar
对于使用人颌骨膜细胞（JPC）的骨再生结构，不同三维支架的骨诱导能力程度尚未确定。我们使用人类 JPC 分析了开孔聚乳酸 (OPLA) 支架作为骨工程结构的适用性。通过扫描电子显微镜观察支架表面的细胞粘附和扩散。将 OPLA 支架内的 JPC 增殖与胶原蛋白和磷酸钙支架内的增殖进行比较。我们发现 OPLA 支架与 Coll/CaP 支架相比，在三个时间点的增殖率显着增加。细胞接种支架内耗氧量的实时测量表明体外培养时间不应超过12-15天。 OPLA 支架被证明对 JPC 生长最有利，被选择用于有或没有 BMP-2 的成骨分化实验。基因表达分析表明，体外培养 12 天后，3D 支架内诱导了多种成骨基因（碱性磷酸酶、osterix、Runx-2 和胰岛素样生长因子）。通过 EDX 光谱法对成骨过程中出现的结节进行元素分析表明，在 OPLA 支架内生长的 JPC 能够形成 CaP 颗粒。我们得出的结论是，OPLA 支架为使用人类 JPC 的骨替代物提供了一个有前景的环境。
For bone regeneration constructs using human jaw periosteal cells (JPC) the extent of osteoinductive ability of different three-dimensional scaffolds is not yet established. We analyzed open-cell polylactic acid (OPLA) scaffolds for their suitability as bone engineering constructs using human JPC. Cell adhesion and spreading was visualized on the surface of scaffolds by scanning electron microscopy. JPC proliferation within OPLA scaffolds was compared with proliferation within collagen and calcium phosphate scaffolds. We found a significant increase of proliferation rates in OPLA scaffolds versus Coll/CaP scaffolds at three time points. Live-measurements of oxygen consumption within the cell-seeded scaffolds indicate that the in vitro culturing time should not exceed 12-15 days. OPLA scaffolds, which were turned out to be the most beneficial for JPC growth, were chosen for osteogenic differentiation experiments with or without BMP-2. Gene expression analyses demonstrated induction of several osteogenic genes (alkaline phosphatase, osterix, Runx-2 and insulin-like growth factor) within the 3D-scaffolds after 12 days of in vitro culturing. Element analysis by EDX spectrometry of arising nodules during osteogenesis demonstrated that JPC growing within OPLA scaffolds are able to form CaP particles. We conclude that OPLA scaffolds provide a promising environment for bone substitutes using human JPC.