Lithographing of Biomolecules on a Substrate Surface Using an Enzyme-Immobilized AFM Tip
Lithographing of Biomolecules on a Substrate Surface Using an Enzyme-Immobilized AFM Tip
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使用固定酶的 AFM 针尖在基材表面上光刻生物分子
DOI：
10.1021/nl034448k
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发表时间：
2003
期刊：
影响因子：
10.8
通讯作者：
J. Miyake
中科院分区：
文献类型：
--
作者：
S. Takeda;C. Nakamura;C. Miyamoto;Noriyuki Nakamura;M. Kageshima;H. Tokumoto;J. Miyake
利用金黄色葡萄球菌丝氨酸V8蛋白水解酶和原子力显微镜(AFM)，在缓冲溶液中成功地进行了酶促光刻。通过破裂酶−底物复合体的力测量，证实了固定化在原子力显微镜针尖上的酶的保留活性。使用酶固定的针尖与底物多肽层接触扫描后，随后的AFM成像清楚地观察到光刻区域的方形形状。
A method of enzymatic lithography was successfully performed in a buffered solution using Staphylococcal serine V8 protease and atomic force microscopy (AFM). The retained activity of the protease immobilized on the AFM tip was confirmed by force measurement to rupture the enzyme−substrate complex. After contact scanning using the enzyme-immobilized tip to the substrate peptide layer, a square shape of the lithographed area was clearly observed by subsequent AFM imaging.