STRUCTURES DERIVED FROM COWPEA CHLOROTIC MOTTLE AND BROME MOSAIC VIRUS PROTEIN
STRUCTURES DERIVED FROM COWPEA CHLOROTIC MOTTLE AND BROME MOSAIC VIRUS PROTEIN
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DOI：
10.1016/0042-6822(69)90374-2
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发表时间：
1969-01-01
期刊：
影响因子：
3.7
通讯作者：
WAGNER, GW
中科院分区：
文献类型：
--
作者：
BANCROFT, JB;BRACKER, CE;WAGNER, GW
研究了三种球形植物病毒分离蛋白在不同盐度和酸性条件下的行为。蚕豆斑驳病毒蛋白没有聚集成可识别的有组织的结构。雀麦花叶病毒的蛋白质在pH和盐依赖的条件下形成与病毒相似的衣壳，在0.2MNaCl中pH为5.0时产量最高。豌豆褪绿斑驳病毒衣壳蛋白在0.1MNaC l溶液中的最适pH为5.0，在0.2MNaC l中的最适pH为3.5~5.0。后一种病毒的蛋白在pH 5.3~5.7的0.2MNaC l中也形成了双壳和玫瑰花环状颗粒。在pH为6.0或更高的条件下制成窄管，如果存在完全消化的RNA，则伴随有t=1和T=3的颗粒。在不含氯化钠的0.01Macetate缓冲液中，在pH为4.0和4.5的条件下，CCMV蛋白呈片状和片状聚集。描述了各种形式的生产和某些性能。
The behavior at different salt and acidic pH levels of protein isolated from three spherical plant viruses was investigated. Broad bean mottle virus protein did not aggregate into recognizable organized structures. Protein from brome mosaic virus formed capsids similar to those of the virus at a pH and salt-dependent efficiency with best yields at pH 5.0 in 0.2MNaCl. Best yields for cowpea chlorotic mottle virus protein capsids occurred at pH 5.0 in 0.1MNaCl or from pH 3.5 to 5.0 in 0.2MNaCl. Protein from the latter virus also formed double-shelled and rosettelike particles in 0.2MNaCl from pH 5.3 to 5.7. Narrow tubes were made at pH levels of 6.0 and higher, and the tubes were accompanied byT= 1 andT= 3 particles if ribonu-clease-digested RNA was present. In the absence of NaCl in 0.01Macetate buffer at pH 4.0 and 4.5, laminar and platelike aggregates were found with CCMV protein. The production and some of the properties of the various forms are described.