Real-time RT-PCR on SAG1 and BAG1 gene expression during stage conversion in immunosuppressed mice infected with Toxoplasma gondii Tehran strain.
Real-time RT-PCR on SAG1 and BAG1 gene expression during stage conversion in immunosuppressed mice infected with Toxoplasma gondii Tehran strain.
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DOI：
10.3347/kjp.2012.50.3.199
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发表时间：
2012-09
期刊：
影响因子：
--
通讯作者：
Keshavarz H
中科院分区：
文献类型：
--
作者：
Selseleh M;Modarressi MH;Mohebali M;Shojaee S;Eshragian MR;Selseleh M;Azizi E;Keshavarz H
弓形虫脑炎是由免疫功能低下的宿主体内的顶复体寄生虫弓形虫的缓殖子重新激活为快速分裂的速殖子引起的。这种危及生命的疾病的诊断是有问题的，因为很难区分这两个阶段。使用gDNA作为模板的弓形虫PCR测定法已经不能区分活性速殖子阶段和潜伏缓殖子阶段之间SAG 1和BAG 1表达的增加或减少。本研究采用实时荧光定量RT-PCR方法检测了T.地塞米松磷酸钠（DXM）给药后的弓形虫德黑兰株。在实验小鼠的肺和脑组织中观察到转化反应，在DXM给药后第6天通过SAG 1表达表示，并持续到第14天。在整个研究期间，在两个器官中也检测到缓殖子;然而，在第14天显著减少。提示缓殖子在复活期不仅能以速殖子的形式从包囊中逃逸并重新侵入邻近细胞，而且还能转化为新的缓殖子。总之，实时荧光定量RT-PCR方法提供了一种可靠、快速、定量的检测T。在动物模型中的弓形虫再激活。因此，该方法可能有助于诊断免疫功能低下患者（艾滋病毒或移植患者）临床标本的分期转换，以早期识别速殖子-缓殖子分期转换。
Toxoplasmic encephalitis is caused by reactivation of bradyzoites to rapidly dividing tachyzoites of the apicomplexan parasite Toxoplasma gondii in immunocompromised hosts. Diagnosis of this life-threatening disease is problematic, because it is difficult to discriminate between these 2 stages. Toxoplasma PCR assays using gDNA as a template have been unable to discriminate between an increase or decrease in SAG1 and BAG1 expression between the active tachyzoite stage and the latent bradyzoite stage. In the present study, real-time RT-PCR assay was used to detect the expression of bradyzoite (BAG1)- and tachyzoite-specific genes (SAG1) during bradyzoite/tachyzoite stage conversion in mice infected with T. gondii Tehran strain after dexamethasone sodium phosphate (DXM) administration. The conversion reaction was observed in the lungs and brain tissues of experimental mice, indicated by SAG1 expression at day 6 after DXM administration, and continued until day 14. Bradyzoites were also detected in both organs throughout the study; however, it decreased at day 14 significantly. It is suggested that during the reactivation period, bradyzoites not only escape from the cysts and reinvade neighboring cells as tachyzoites, but also converted to new bradyzoites. In summary, the real-time RT-PCR assay provided a reliable, fast, and quantitative way of detecting T. gondii reactivation in an animal model. Thus, this method may be useful for diagnosing stage conversion in clinical specimens of immunocompromised patients (HIV or transplant patients) for early identification of tachyzoite-bradyzoite stage conversion.
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影响因子：
3
作者：
Mesquita, Rafael T.;Ziegler, Angela P.;Pereira-Chioccola, Vera L.
通讯作者：
Pereira-Chioccola, Vera L.
影响因子：
2.7
作者：
Wittwer, CT;Herrmann, MG;Rasmussen, RP
通讯作者：
Rasmussen, RP
影响因子：
3.3
作者：
FRENKEL, JK;NELSON, BM;ARIASSTELLA, J
通讯作者：
ARIASSTELLA, J
影响因子：
9.4
作者：
Joseph, P;Calderón, MM;Evans, CA
通讯作者：
Evans, CA
影响因子：
--
作者：
Kang, Ki-Man;Choi, In-Uk;Lee, Young-Ha
通讯作者：
Lee, Young-Ha