Effects of probiotic and Bidens pilosa on the performance and gut health of chicken during induced Eimeria tenella infection
Effects of probiotic and Bidens pilosa on the performance and gut health of chicken during induced Eimeria tenella infection
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益生菌和鬼针草对诱导柔嫩艾美耳球虫感染期间鸡的生产性能和肠道健康的影响
DOI：
10.1111/jam.14928
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发表时间：
2020-12-03
影响因子：
4
通讯作者：
Si, H.
中科院分区：
文献类型：
--
作者：
Memon, F. Q.;Yang, Y.;Si, H.
目的研究益生菌和鬼针草对鸡柔嫩艾美耳球虫（Eimeria tenella）感染期间的生产性能和肠道健康的单独和联合保护机制。方法和结果将150只1日龄鸡平均分为5个处理组，每个处理组设3个生物学重复。两组被分配为对照组（对照组未处理未攻击，CG和对照阳性未处理攻击，CPG），三组被喂食含有益生菌（PG）、B的饮食。pilosa（BPG）和益生菌+B。pilosa（PG + BPG）感染E. tenella。检测各组鸡感染前后体重、卵囊排出量、盲肠病变评分、凋亡相关蛋白（Bcl-2、Bax和caspase-3）、抗氧化酶（CAT和SOD 1）、促炎细胞因子（IL-6和IL-8）和紧密连接蛋白（CLDN 1和ZO 1）mRNA表达水平。结果表明，在感染过程中（21 - 29天），E.与未感染的对照鸡相比，tenella攻击的鸡的体重显著降低;然而，用益生菌B喂养的鸡的体重没有显著影响。pilosa和益生菌+B。观察到有毛。柔嫩艾美耳球虫攻击未处理的鸡与处理的攻击鸡相比，卵囊脱落、盲肠破坏率和死亡率增加（P0中心点05）。与PG、BPG和PG + BPG组相比，CPG组抗凋亡蛋白Bcl-2 mRNA表达上调，促凋亡蛋白Bax mRNA表达下调。此外，饲喂益生菌的鸡，B。pilosa和益生菌+B。结论益生菌和B. pilosa（单独或组合）似乎在抑制疾病的发生和降低鸡艾美耳球虫感染的严重性方面是有效的。研究的意义和影响本研究解释了益生菌和B. pilosa（单独和/或组合）改善鸡的生产性能并防止由E. tenella。
Aim In this study, we have examined the individual and combined protective mechanism of probiotic and Bidens pilosa on the performance and gut health of chickens during Eimeria tenella infection over a 29-day experimental trial.Methods and Results A total of one hundred and fifty 1-day-old chickens were equally distributed into five treatment groups with three biological replicates: two groups were allocated as control groups (control group untreated unchallenged, CG and control positive untreated challenged, CPG) and three groups were fed diets with probiotic (PG), B. pilosa (BPG) and probiotic + B. pilosa (PG + BPG) and challenged with E. tenella. Birds of all groups were assessed for pre and post-infection body weights, oocysts shedding, caecal lesion scores and mRNA expression levels of apoptosis related proteins (Bcl-2, Bax and caspase-3), antioxidant enzymes (CAT and SOD 1), pro-inflammatory cytokines (IL-6 and IL-8) and tight junction proteins (CLDN 1 and ZO 1). Our results revealed that during infection (day 21-29), E. tenella challenged chickens significantly decreased the body weight compared with uninfected control chickens; however, there was no significant effect on body weight of chickens fed with probiotic, B. pilosa and probiotic + B. pilosa was observed. Eimeria tenella challenged untreated birds increased (P 0 center dot 05) oocysts shedding, destructive ratio of caeca and mortality as compared to treated challenged birds. CPG group up-regulated the mRNA expression levels of anti-apoptosis protein Bcl-2 while down-regulated the pro-apoptosis protein Bax relative to PG, BPG and PG + BPG groups. Moreover chickens fed probiotic, B. pilosa and probiotic + B. pilosa diets enhanced the activities of antioxidant enzymes, pro-inflammatory cytokines and tight junction proteins with the comparison of control positive untreated challenged chickens.Conclusion These findings elaborated that feed supplementation of probiotic and B. pilosa (individually or in combination) appeared to be effective in inhibiting the occurrence of disease and decreasing the severity of Eimeria infection in chickens.Significance and Impact of the Study This study explained the underlying anti-coccidial mechanism in which probiotic and B. pilosa (individually and/or in combination) improve the performance of chicken and protect against gut inflammatory responses caused by E. tenella.