FADS gene polymorphisms in Koreans: Association with ω6 polyunsaturated fatty acids in serum phospholipids, lipid peroxides, and coronary artery disease
FADS gene polymorphisms in Koreans: Association with ω6 polyunsaturated fatty acids in serum phospholipids, lipid peroxides, and coronary artery disease
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DOI：
10.1016/j.atherosclerosis.2010.10.004
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发表时间：
2011-01-01
期刊：
影响因子：
5.3
通讯作者：
Lee, Jong Ho
中科院分区：
文献类型：
--
作者：
Kwak, Jung Hyun;Paik, Jean Kyung;Lee, Jong Ho
目的：研究韩国人FADS基因多态性与血清磷脂、脂质过氧化物和冠状动脉疾病（CAD）中多不饱和脂肪酸（PUFAs）的关系。方法：在本病例对照研究中，CAD患者（n=756, 40-79岁）和健康对照（n=890）分别在FADS1 （FEN1 rs174537G>T）、FADS2 （rs174575、rs2727270）和FADS3 （rs1000778）附近进行rs174537基因分型。我们计算了冠心病风险的优势比（ORs），并测量了血清PUFA组成和过氧化脂质。结果：校正年龄、BMI、吸烟、饮酒、高血压、糖尿病、高脂血症等因素后，4个snp中，对照组与冠心病患者之间只有rs174537G>T等位基因频率存在差异（P=0.017）。校正后，较小的T等位基因与较低的CAD风险相关[OR 0.75 (95% CI 0.61-0.92)， P=0.006]。rs174537T携带者的亚油酸（LA, 18:2 ω - 6）比例显著高于对照组和CAD组，花生四烯酸（AA, 20:4 ω - 6）比例显著低于对照组和CAD组，AA/二同型γ -亚麻酸（DGLA, 20:3 ω - 6）和AA/LA比例显著低于G/G组。在对照组中，174537T携带者的总胆固醇和低密度脂蛋白、丙二醛和ox-LDL水平显著降低。在CAD患者中，rs174537T携带者的LDL颗粒尺寸比G/G组大。血清磷脂中AA的比例与对照组的ldl -胆固醇、ox-LDL和丙二醛呈正相关，与对照组和CAD组的8-epi-前列腺素F-2 α呈正相关。结论：rs174537T与血清磷脂中AA比例降低、冠心病风险降低有关，与总胆固醇、低密度脂蛋白胆固醇和脂质过氧化物降低有关。爱思唯尔爱尔兰有限公司版权所有版权所有。
Objective: We investigated the association of polymorphisms in FADS genes with polyunsaturated fatty acids (PUFAs) in serum phospholipids, lipid peroxides, and coronary artery disease (CAD) in Koreans.Methods: In this case-control study, CAD patients (n=756, 40-79 years) and healthy controls (n=890) were genotyped for rs174537 near FADS1 (FEN1 rs174537G>T), FADS2 (rs174575, rs2727270), and FADS3 (rs1000778). We calculated the odds ratios (ORs) for CAD risk and measured serum PUFA composition and lipid peroxide.Results: Among four SNPs, only rs174537G>T differed in allele frequencies between controls and CAD patients after adjustment for age, BMI, cigarette smoking, alcohol consumption, hypertension, diabetes mellitus, and hyperlipidemia (P=0.017). The minor T allele was associated with a lower risk of CAD [OR 0.75 (95% CI 0.61-0.92), P=0.006] after adjustment. rs174537T carriers had a significantly higher proportion of linoleic acid (LA, 18:2 omega 6), lower arachidonic acid (AA, 20:4 omega 6), and lower ratios of AA/dihomo-gamma-linolenic acid (DGLA, 20:3 omega 6) and AA/LA than G/G subjects in both control and CAD groups. In the control group, 174537T carriers had significantly lower levels of total- and LDL-cholesterol, malondialdehyde, and ox-LDL. In CAD patients, rs174537T carriers showed a larger LDL particle size than G/G subjects. The proportion of AA in serum phospholipids positively correlated with LDL-cholesterol, ox-LDL, and malondialdehyde in controls and with 8-epi-prostaglandin F-2 alpha in both control and CAD groups.Conclusion: The rs174537T is associated with a lower proportion of AA in serum phospholipids and reduced CAD risk, in association with reduced total-and LDL-cholesterol and lipid peroxides. Crown Copyright (C) 2010 Published by Elsevier Ireland Ltd. All rights reserved.