Effect of thyroparathyroidectomy on the activities of thymidylate synthetase and thymidine kinase during liver regeneration after partial hepatectomy.
Effect of thyroparathyroidectomy on the activities of thymidylate synthetase and thymidine kinase during liver regeneration after partial hepatectomy.
复制标题
甲状腺甲状旁腺切除术对肝部分切除术后肝再生过程中胸苷酸合成酶和胸苷激酶活性的影响。
DOI：
10.1042/cs0720455
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发表时间：
1987
期刊：
影响因子：
6
通讯作者：
S. Kojo
中科院分区：
文献类型：
--
作者：
R. Nakata;I. Tsukamoto;M. Miyoshi;S. Kojo
甲状腺甲状旁腺切除术（TPTX）进行前72小时部分肝切除术（PH）减少诱导的肝胸苷酸合成酶（TS）和胸苷激酶（TK），这是在DNA合成的速率决定酶，在PH后24小时。当TPTX进行前24小时PH，TK活性在PH后24小时并没有减少，但TS活性显着降低。因此，TPTX的作用在TS和TK之间的时间依赖性不同。在PH前72 h给予TPTX后，大鼠TK活性下降，补充Ca ~（2+）后，TK活性恢复。这一结果表明，再生肝中TK活性的升高受血浆Ca ~（2+）的调节。由于需要高剂量的三碘甲状腺原氨酸（T3）引起升高这些酶的活性和DNA含量在24小时再生肝TPTX大鼠，T3的相对贡献可能是小的。
Thyroparathyroidectomy (TPTX) carried out at 72 h before partial hepatectomy (PH) reduced the induction of hepatic thymidylate synthetase (TS) and thymidine kinase (TK), which are rate-determining enzymes in DNA synthesis, at 24 h after PH. When TPTX was carried out at 24 h before PH, TK activity at 24 h after PH was not reduced at all, yet TS activity was reduced significantly. Thus the effect of TPTX differed in time dependence between TS and TK. The depression of TK activity in rats which were subjected to TPTX at 72 h before PH, was recovered by Ca2+ supplementation. This result demonstrated that the rise of TK activity in regenerating liver is regulated by plasma Ca2+. Since a high dose of tri-iodothyronine (T3) was required to cause elevation of the activities of these enzymes and DNA content in 24 h-regenerating liver of TPTX rats, the relative contribution of T3 to liver regeneration may be small.