Genotoxic effects of Israeli industrial pollutants on residents of Bruqeen village (Salfit district, Palestine)
Genotoxic effects of Israeli industrial pollutants on residents of Bruqeen village (Salfit district, Palestine)
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以色列工业污染物对 Bruqeen 村（巴勒斯坦萨尔菲特区）居民的基因毒性影响
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发表时间：
2013
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影响因子：
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通讯作者：
M. Qumsiyeh
中科院分区：
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作者：
Khloud M. Hammad;M. Qumsiyeh
许多工业副产品是遗传毒性剂，诱导细胞遗传学变化和DNA损伤。Bruqeen是西岸北方萨尔菲特区的一个巴勒斯坦村庄，受到来自Barqan以色列工业定居点的工业废料的影响。我们使用来自试验和对照部位的全血样本评价了诱导人细胞的染色体断裂（Csb）和DNA损伤程度。通过常规细胞遗传学方法评估Csb，并通过彗星试验（也称为单细胞凝胶电泳）评估DNA损伤。对30例Bruqeen居民的细胞遗传学分析显示：133例出现着丝粒早分离（PCS），43例出现Csb，40例出现染色单体断裂（Ctb），26例出现双着丝粒。总的来说，结果显示平均4.08%的染色体畸变（CA）和3.81%的CA细胞。对照组（Bethlehem地区n = 8）检出PCS 21例，Csb 2例，Ctb 5例，双着丝粒2例，平均CA 1.97例，CA细胞1.91%。统计分析表明，暴露受试者和对照受试者之间的PCS、Ctb和双着丝粒细胞无显著差异（p值> 0.05），但具有CA和Csb的细胞的CA频率存在统计学显著差异（p值< 0.05）。25名布鲁金居民的彗星分析数据显示，与对照样本的数据有显著差异（p值< 0.05）。
Many industrial byproducts are genotoxic agents that induce cytogenetic changes and DNA damage. Bruqeen, a Palestinian village in Salfit district in the northern West bank, is subject to industrial waste products coming from Barqan Israeli industrial settlement. We evaluated the extent of chromosomal breaks (Csb) and DNA damage induced to human cells using whole blood samples from both test and control sites. Csb were assessed by routine cytogenetic methods and DNA damage was assessed via the Comet assay which is also called single cell gel electrophoresis. Cytogenetic analysis for Bruqeen residents’ sample (n = 30) showed 133 Premature centromere separation (PCS), 43 Csb, 40 chromatid breaks (Ctb) and 26 dicentrics. In total, the findings showed an average of 4.08% chromosomal aberrations (CA) and 3.81% cells with CA. The results of controls (from Bethlehem area n = 8) showed 21 PCS, 2 Csb, 5 Ctb and 2 dicentric, and in total the average showed 1.97 CA and 1.91% cells with CA. Statistical analysis showed that there were no significant differences between exposed and control subjects for PCS, Ctb and Dicentric (p-value > 0.05), but there was a statistically significant difference for CA frequency for cells that have CA and Csb (p-value < 0.05). Comet assay data on the 25 Bruqeen residents showed that there was a significant difference from those of the control samples (p-value < 0.05).