mp23, a Theileria parva transmembrane protein with homology to the protein disulfide isomerase family
mp23, a Theileria parva transmembrane protein with homology to the protein disulfide isomerase family
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DOI：
10.1016/s0166-6851(02)00036-1
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发表时间：
2002-05-01
影响因子：
1.5
通讯作者：
Lipp, J
中科院分区：
文献类型：
--
作者：
Ebel, T;Bender, K;Lipp, J
为了识别。筛选了200个可在内质网（ER）衍生微粒体膜（rough microsomes， RM）上易位的编码分泌蛋白和膜蛋白的补体dna （parvaccomplementarydna, cDNA）。发现17号克隆转录的T7 RNA聚合酶启动子与RM交叉。序列分析显示，克隆17全长755 bp，包含一条28 bp的poly-A尾部。在RM的存在下合成了一个额外的23 kDa的产物，称为mp23。克隆17只有一个开放阅读框的长度足以编码大于14 kDa的多肽。从第一个ATG密码子开始，产生220个氨基酸的多肽，称为p25，计算分子质量为24 682 Da。推测mp23是一种跨膜蛋白，具有一个跨膜片段和一个短的c端胞浆尾部。通过数据库检索，发现p25的n端区域与蛋白二硫异构酶（PDI）家族成员的氧化还原活性硫氧还蛋白同源结构域具有显著的相似性。基于完整的pdi -硫氧还蛋白同源片段的存在及其信号肽介导的进入er源性膜囊腔的易位，parvamp23被认为是pdi相关蛋白。报告的核苷酸序列数据可从EMBL数据库获得，登录号为AJ300678。
In order to identifyT. parvacomplementary DNAs (cDNA) encoding secretory and membrane proteins, which can be translocated across enodoplasmic reticulum (ER)-derived microsomal membranes (rough microsomes (RM)), 200 individual clones were screened. It was found that clone 17 transcribed the T7 RNA polymerase promoter crosses RM. Sequence analysis revealed that clone 17 comprises a cDNA of 755 bp including a 28 bp poly-A tail. Synthesis in the presence of RM led to an additional product of 23 kDa, termed mp23. Only one open reading frame of clone 17 had sufficient length to encode a polypeptide larger than 14 kDa. Starting with the first ATG codon the resulting in 220 amino acid polypeptide, termed p25, had a calculated molecular mass of 24 682 Da. It is deduced that mp23 is a transmembrane protein, with a single membrane-spanning segment and a short C-terminal cytosolic tail. Performing database searches, it was found that the N-terminal region of p25 shows significant similarity to redox-active thioredoxin homology domains of members of the protein disulfide isomerase (PDI) family. Based on the presence of a complete PDI-thioredoxin homology segment and its signal peptide-mediated translocation into the lumen of ER-derived membrane vesicles,T. parvamp23 is regarded as PDI-related protein. Nucleotide sequence data reported are available from the EMBL database under accession number AJ300678.