Downregulation of Notch1 induces apoptosis and inhibits cell proliferation and metastasis in laryngeal squamous cell carcinoma
Downregulation of Notch1 induces apoptosis and inhibits cell proliferation and metastasis in laryngeal squamous cell carcinoma
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Notch1下调诱导喉鳞状细胞癌细胞凋亡并抑制细胞增殖和转移
DOI：
10.3892/or.2015.4274
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发表时间：
2015-12-01
期刊：
影响因子：
4.2
通讯作者：
Chen, Shi-Ming
中科院分区：
文献类型：
--
作者：
Dai, Meng-Yuan;Fang, Fang;Chen, Shi-Ming
Noch信号在多种人类肿瘤中起着关键作用；它可以是癌基因，也可以是肿瘤抑制基因，具体取决于组织环境。Notch1在喉鳞状细胞癌(LSCC)中的功能目前尚不清楚。我们研究了Notch1在LSCC细胞生长、凋亡和转移中的作用。我们用量子点免疫组织化学方法(QD-IHC)和常规免疫组织化学方法分析了Notch1在临床喉癌组织中的表达。将Notch1特异性短发夹状RNA(ShRNA)导入人喉癌Hep-2细胞，分别用细胞计数法、流式细胞仪、伤口愈合和Transwell法检测细胞增殖、凋亡和迁移侵袭能力，Western blotting检测Notch1靶基因的表达。与正常喉鳞状细胞癌组织相比，Notch1在喉癌组织中表达上调，在有转移的喉癌组织中表达增强(P
Notch signaling plays a key role in a wide variety of human tumors; it can be an oncogene or a tumor-suppressor gene depending on the tissue context. The functions of Notch1 in laryngeal squamous cell carcinoma (LSCC) are largely unknown. We investigated the role of Notch1 in LSCC cell growth, apoptosis and metastasis. We analyzed Notch1 expression in clinical LSCC samples using quantum dot immunohistochemistry (QD-IHC) and conventional IHC. Human laryngeal carcinoma HEp-2 cells were transfected with Notch1-specific short hairpin RNA (shRNA), and cell proliferation, apoptosis, and migration and invasion were evaluated using the cell counting assay, flow cytometry and wound healing and Transwell assays, respectively; western blotting was used to validate the expression of Notch1 target genes. Compared with normal tissues, Notch1 was upregulated in LSCC tissues; compared with LSCC tissues without metastasis, Notchl upregulation was enhanced in LSCC tissues with metastasis (P